Experimental Studies On BMP2-immobilized PFM Scaffolds For Osteo-differentiation Of Adipose-derived Stem Cells

ObjectivesIn order to find a new type of bone tissue engineering scaffold for bone defect repair,this study will investigate the effects of biologically active polymer materials PFM and PFM/BMP2 sustained-release scaffold on the vitality,proliferation,osteogenic differentiation of adipose-derived mesenchymal stem cells(ADSCs)and the repair of critical-sized calvarial bone defect in rats for establishing the experimental basis for the application of PFM in bone defect repair.Methods1.The effect of PFM on the vitality,proliferation and osteogenic differentiation of rADSCs was detected by LDH assay,BrdU assay,qPCR and immunofluorescence staining in vitro.The effect of PFM on the repair of critical-sized calvarial bone defect in rat was also observed.2.The effect of PFM/BMP2 sustained-release scaffolds on the osteogenic differentiation of rADSCs was detected by qPCR,alkaline phosphatase staining and immunofluorescence staining.The rat critical-sized calvarial bone defect was implanted with PFM/BMP2 sustained-release scaffolds combined with rADSCs and the effect of the complex on the repair of the defect was also observed.Results1.PFM is rich in free hydroxyl and carboxyl chemical groups;PFM can promote the proliferation and osteogenic differentiation of rat adipose mesenchymal stem cells;PFM can promote the repair of rat critical-sized calvarial bone defect.2.PFM/BMP2 sustained-release scaffolds could effectively and steadily release BMP2.PFM/BMP2 sustained-release scaffolds could promote the osteogenic differentiation of rat adipose-derived mesenchymal stem cells.PFM sustained-release scaffold could promote the healing of critical-sized calvaria bone defect in rats.Conclusion1.Bioactive polymer material PFM has excellent biocompatibility,can promote the proliferation and osteogenic differentiation of rat adipose mesenchymal stem cells,as well as the repairing effect of rat critical-sized calvaria bone defect,suggesting that the bioactive polymer material PFM has potential application value in bone tissue engineering.2.Construction of PFM/BMP2 sustained-release scaffolds by covalently coupling PFM porous scaffolds and BMP2 could promote the osteogenic differentiation of adiposederived mesenchymal stem cells and the repairing effect of critical-sized calvaria bone defect in rat skull,suggesting that PFM/BMP2 sustained-release scaffolds has a great potential application value in the treantment of bone defect.