The Expression Of LncRNA TPT1-AS1 In Different Gastric Diseases And Its Correlation With Gastric Intestinal Metaplasia Induced By HpSlyD

Objective: To explore the differential expressions of TPT1-AS1 among different gastric disease tissues(basically normal、atrophic gastritis and gastric carcinoma).To analyze the correlation between the expression levels and the clinicopathologic features of gastric cancer.To evaluate the correlation between the expression levels and the prognosis of patients with gastric cancer.And to explore its relevance with gastric metaplasia induced by HpSlyD.Methods: 1.Employ the method of q PCR(quantitative real-time PCR)to detect the expression levels of TPT1-AS1 in tumorous tissues(73 examples)、atrophic gastritis tissues(25 examples)as well as the distal normal tissues(66 examples).Independent sample T-test was employed to analyze the differential expressions among the tumorous tissues、the atrophic gastritis tissues and the distal normal tissues.Analyze its correlation with clinical pathological characteristic of gastric carcinoma with independent sample T-test or univariate analysis.Survival analysis was performed with Kaplan-Meier method to determine the correlation between TPT1-AS1 and prognosis of gastric cancer patients;2.Employ the method of q PCR to detect the expression levels of CDX2 m RNA in the atrophic gastritis tissue,and Spearman analysis was employed to analyze the correlation between TPT1-AS1 and CDX2 m RNA expressions;3.HpSlyD was employed at the concentration of 200ng/ml to stimulate AGS cell line for 40 hours.Then extract the total proteins and total RNAs,and employ Western Blot(WB)to detect the expression levels of proteins in CDX2 and Villin,employ q PCR to detect the expression levels of RNAs in CDX2 and Villin as well;4.HpSlyD at the concentration of 200ng/ml and FK506 at the concentration of 18mg/ml were employed to stimulate AGS cell line for 40 hours.Then extract the total proteins and total RNAs,and employ WB to detect the expression levels of proteins in CDX2 and Villin,employ q PCR to detect the expression levels of RNAs in TPT1-AS1,CDX2 and Villin as well.Results: 1.The expression level of TPT1-AS1 was elevated in atrphic gastritis tissues,while reduced in gastric carcinoma tissues;2.The expression level of TPT1-AS1 was unrelated with the clinicopathologic feature of gastric carcinoma;3.TPT1-AS1 was not associated with overall survival in patients with gastric cancer;4.In the atrophic gastritis,the expression level of TPT1-AS1 was positively related to the expression level of CDX2 m RNA;5.In the metaplasia of AGS cell lines induced by HpSlyD,the expression level of TPT1-AS1 was increased;6.FK506,HpSlyD binding protein,can block the metaplasia function of AGS cell lines induced by HpSlyD,during which process block the expression of TPT1-AS1 as well.Conclusion: 1.TPT1-AS1 is high expressed in atrophic gastritis tissues and low expressed in gastric carcinoma tissues;2.TPT1-AS1 is not associated with clinicopathologic feature and overall survival of patients with gastric cancer;3.TPT1-AS1 is related to the gastric metaplasia induced by HpSlyD.