Effect Of S100A4 On The Biological Behavior Of Human Gastric Cancer Cells And Its Mechanism

Objective:Gastric cancer is a highly heterogeneous malignant tumor.The cell metabolism process involved in its development is a complex process involving multiple stages,multiple genes,and multiple factors.The abnormal expression and distribution of S100A4 protein in the development of human gastric cancer plays an important role.However,its specific effect mechanism is not yet fully clear.Therefore,in this paper,we propose to establish S100A4 gene silencing human gastric cancer SGC-7901,MGC-803 cells to detect the effect of S100A4 gene silencing on changes in intracellular calcium ion concentration and related protein expression.At the same time,the resulting changes in c-jun and c-fos gene expression and malignant biological behavior of human gastric cancer cells were detected,and the effects of S100A4 on the biological behavior of human gastric cancer cells and its mechanism were initially discussed.Methods:1,Human gastric cancer SGC-7901,MGC-803 cell culture:cells are cultured in a cell medium containing 10%superior fetal serum and placed in a 37°C thermostat containing 5%CO₂ for culture,change the culture fluid every three days.2,Establish S100A4 gene silent human stomach cancer cells:use lip2000 to transfer siRNA-NC and siRNA-S100A4 to human stomach cancer cells SGC-7901 and MGC-803.3,Effects of S100A4 gene silencing on intracellular calcium concentration and expression of S100A4,PKC-?,and CaM proteins:Changes in free calcium concentration in human gastric cancer cells were measured by fluorescence probe Fura 2-AM,and Western blot detected changes in related protein expression.4,Effects of S100A4 gene silencing on expression of S100A4,c-jun and c-fos genes and malignant biological behavior of human gastric cancer cells:changes in expression of S100A4,c-jun and c-fos genes were detected by real time fluorescence quantitative PCR?qPCR?;The cell proliferation ability of malignant biological behavior was determined by MTT experiment,cell cloning experiment and apoptosis experiment;Changes in invasions and metastases were detected by cell scratches and transspell experiments.5,The expression of S100A4,PKC-?,CaM and c-jun,c-fos in gastric cancer was statistically analyzed using SPSS17.0 software.The main statistical method uses t test?or variance analysis?,P<0.05 indicates that the difference is statistically significant,and P>0.05 indicates that the difference is not statistically significant.Results:1,Successfully established S100A4 gene silent human gastric cancer SGC-7901,MGC-803 cells.2,S100A4 gene silencing SGC-7901,MGC-803 cells,S100A4 protein expression and intracellular free calcium ion concentration decreased,which in turn caused the expression of calcium ion-related proteins PKC-?and CaM decreased.3,After the S100A4 gene is silent,the decreased expression of PKC-?protein will affect the expression of c-jun and c-fos in the nucleus,which will lead to a significant decrease in the proliferation,invasion,and transfer ability of SGC-7901 and MGC-803 cells and induceapoptosis.Conclusion:S100A4 can affect the malignant biological behavior of gastric cancer cells by affecting the concentration of calcium ions in cells,which in turn regulates the expression of PKC-?,CaM and c-jun,c-fos.These results show that S100A4 may have the potential to become a target for the treatment of gastric cancer.