Detection Of Cerebral Cortical Vessels And Anterior Chamber Angle Of Human Eye Based On Optical Coherence Tomography

Modern social cerebrovascular diseases and glaucoma are important diseases that endanger human life and health in today's world,and are crucial for the study of cerebrovascular imaging technology and anterior chamber angle imaging.The paper introduces various methods of traditional cerebrovascular imaging and human eye imaging methods,which have the disadvantages of contact or inability to depth imaging or low resolution,while optical coherence tomography(OCT)has non-contact.The advantages of high resolution,fast acquisition speed,and depth resolution are not only able to display the three-dimensional structure of the sample,but also to detect motion information in the sample.The system designed in this paper uses spectral OCT to construct a high-resolution spectral OCT imaging system based on fiber optic path using spectral OCT principle.The system has a lateral resolution of 6.7 microns and a vertical resolution of 4.7 microns,using a 140 k line/second camera line.Acquisition,can complete 3D scanning in 2 seconds.Aiming at the system,this paper designed a method based on the principle of dispersion and spectral processing,and wrote a control acquisition program based on Lab VIEW software.The program has real-time imaging,removing mode noise,controlling scanning galvanometer and high-speed acquisition.This system was used to scan the three-dimensional vascular network of the rat cerebral cortex.In the rat brain,the OCT-based depth imaging principle obtained 3D cerebrovascular images by XY plane scanning,and the 12 small-scale scanned images were spliced and synthesized into 12mm×8mm images by the correlation of adjacent boundaries,and the imaging results were not lost.Resolution and clear resolution of capillaries provide theoretical basis and operational experience for OCT's ability to resolve human cerebrovascular imaging.The system also studied the anterior chamber angle of the human eye.The full-eye scan imaging method of the X-Y plane was used to acquire and restore the 360° full-angle 3D image,and the imaging method of the multi-azimuth scanning anterior chamber angle was proposed.The results of X-Y plane full-angle scanning method and multi-azimuth scanning method are compared.The results show that the multi-azimuth scanning method has smaller variance and faster speed.Our results show that the system has high spatial resolution and contrast,enabling realtime dynamic three-dimensional imaging of rat cerebral vessels and anterior chamber angles of the human eye.This research lays a solid foundation for the clinical application of spectral OCT technology.