| Background Diabetic nephropathy is a chronic kidney disease caused by diabetes,and its number of patients is increasing year by year.Increasing evidence suggests that inflammation plays an important role in the pathogenesis of DN.As an anti-inflammatory cytokine,IL-10 can limit the tissue damage caused by inflammation,current its exact molecular mechanism is unclear,and epigenetic mechanisms of DNA methylation is involved in the regulation of inflammatory genes related to Diabetic nephropathy,whether the expression of IL-10 is intrinsically linked to DNA methylation is a matter of concern.Objectives To explore the relationship between the express of interleukin-10 and DNA methylation in renal tissues of rats with type 2 Diabetic nephropathy,and to provide a theoretical basis for development a new anti-inflammatory treatment strategy for diabetic nephropathyMethods 48 clear grade healthy male SD rats,weighing 160-200 g,aged 5-8 weeks,were free to drink and eat.SD rats were randomly classified as normal control group(NC group,n=24)and diabetes group(DM group,n=24).The diabetic model was induced by intraperitoneal injection of 1% streptozotocin at 40mg/kg.After 72 hours and 1 week,the tail vein bloods samples were collected and the random blood glucose was higher than 16.7 mmol/L and the two times of positive urine glucose were all successful.After successful modeling,blood glucose?urine glucose?urine microalbumin and urine creatinine index were detected at 0?4?8?12 weeks respectively.At the same time,HE?PAS?Masson and PASM staining were used to observe the histopathological changes of rat kidney at different time points.Tested IL-10 and DNMT1 levels with immunohistochemical method,and the correlation of DN indicators was analyzed.Results 1.Results of general biochemical indicators: Compared with NC group,The modeling rate of rats in the DM group was 75%,while there were significant symptoms of drinking,eating and polyuria,and by 12 weeks the weight of rats in the DM group was(478±79.1)g,significant lower than that of NC group(650.0±26.9)g,the difference was statistically significant;Compared with the NC group,The urinary protein creatinine ratios of the DM group were different at the weekends of 0?8 and 12;After successful modeling,the blood glucose of the DM group,and the blood glucose remained at(23.0±5.5)mmol/L until the end of the experiment,the difference was statistically significant.2.Light microscopy results: There were no obvious abnormalities in the glomeruli?tubules and renal interstitial tissue of rats in the NC group at different times under the light microscope.Compared with the NC group,all kinds of staining under the renal light microscopy in the DM group showed renal interstitial edema and inflammatory cell infiltration.At the 12 th week,the glomerular basement membrane was slightly thickened and vacuolation of renal tubular epithelial cells.3.Immunohistochemical results: IL-10 is mainly express in the cytoplasm of renal tubular epithelial cells,and DNMT1 is mainly express in the nucleus of renal tubular epithelial cells;The expression of IL-10 in renal tissues of NC group was higher than that of DM group in the same period(P<0.05);The expression of DNMT1 in renal tissues of NC group was lower than that of DM group in the same period(P<0.05).4.There was no significant correlation between IL-10 and DNMT1 expression in DN rat renal tissue(r=0.172,P=0.540).Conclusions IL-10 level decreased and DNMT1 level increased in renal tissue of rats with type 2 diabetic nephropathy,but IL-10 expression was not significant correlated with DNMT1 expression,and other factors may be involved. |
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