Mechanism Of Fuzi Lizhong Decoction In Treatment Of Advancedgastriccancerbased On Network Pharmacology

Objective To explore the mechanism of Fuzi Lizhong Decoction in treatment of advanced gastric cancer based on molecular docking and networkMethods Firstly,TCMSP database,Drug Bank database and harmMapper platform were used to screen and predict the chemical constituents in Fuzi Lizhong Decoction,and the related targets of gastric cancer were obtained through OMIM database,GAD database and CTD database.Then,Wein map was made by DrawVenDiagram platform to obtain the intersecting target proteins.Cytoscape 3.5.1 software was used to obtain the target proteins.The protein interaction map was constructed,and then the gene ontology function enrichment analysis and KEGG pathway analysis were carried out from three aspects of biological process(BP),molecular function(MF)and cellular component(CC)by DAVID analysis tool and KOBAS 3.0 software.Finally,PDB database and Systems DockWeb Site were used to analyze the gene ontology function enrichment and KEGG pathway.The database set was used for receptor-ligand molecular docking and docking scores were analyzed to support the rationality ofFuzi Lizhong Decoction in the treatment of advanced gastric cancer.Results 147 chemical components of Fuzi Lizhong Decoction were screened out from TCMSP database,of which 4791 targets were predicted,28006 were screened out by CTD and OMIM,and 346 potential targets were identified by Wayne diagram.The key targets of the interaction between Fuzi Lizhong Decoction and gastric cancer included TP53(tumor suppressor protein p53),AKT1(protein T1 Kinase B,CDKN1A(cyclin dependent kinase inhibitor 1a),STAT3(signal transducer and activator of transcription 3),BCL2L1(survival promoting factor),JUN(intranuclear cancer gene),CCND1(cyclin Dl),EGF(epidermal growth factor),etc.The main mechanisms of their use may involve the 2-oxocarboxylic acid metabolism(2-oxocarboxylic acid metabolism)signal pathway and propanoate.The results of molecular docking showed that STAT3 had the highest docking fraction and had good binding with Fuzi Lizhong decoctionConclusion The key targets of Fuzi Lizhong Decoction in the treatment of advanced gastric cancer include TP53,AKT1,CDKN1A,STAT3,BCL2L1,JUN,CCND1,EGF,etc.It may play a role through biological processes such as cell matrix adhesion,positive regulation of telomerase activity and fatty acid biosynthesis.Its main mechanism may involve molecular pathways such as 2-oxocarboxylic acid metabolism and propanoate metabolism STAT3 has a good combination with Fuzi Lizhong decoction,which may play an important role in the treatment of advanced gastric cancer.