The Correlation Between AGEs And Glucose And Lipid Metabolism In Polycystic Ovary Syndrome

Objective:In order to research the correlation between advanced glycation end products(AGEs)and glucose and lipid Metabolism in polycystic ovary syndrome(PCOS),exploring the development of polycystic ovary syndrome.Methods: All patients of childbearing age(18-40 years old)in my study were from the laparoscopic department and gynaecology department of the First Affiliated Hospital of Dalian Medical university between 2014 and 2019.These patients were admitted to hospital for bariatric surgery or excision of ovarian cyst.we collected their demographic data,including age,occupation,menstrual history,marriage and childbearing history,past history,family history,etc,anthropometric data,including weight,height,body mass index,waist circumference,hip circumference and blood pressure.At the same time,the clinical manifestations of hyperandrogens such as acne,and hirsutism were observed.Before surgery,the patients would take oral glucose tolerance test(OGTT)and insulin release test.Blood samples of all patients were collected before surgery,the upper serum was centrifuged to detect the related clinical biochemical indexes of sex hormone,glucose metabolism,lipid metabolism.And the content of advanced glycation end products(AGEs)in serum was detected by ELISA method.The fat tissue samples of all patients were collected during the operation,fixed with formaldehyde and made into paraffin section,stained with H&E,observed the morphology of fat tissue under microscope,and measured the size of fat cells with Image J software.Fat cell classification standard as follows: area:<5000um2,5000um2~10000um2,≥10000um2;perimeter:<200um,200um~<400um,≥400um;long diameter:<50um,50um~100um≥100um.According to the Rotterdam diagnostic criteria of PCOS,all patients were divided into PCOS group and non PCOS group.The differences appeal indexes between the two groups were compared.The correlation between advanced glycation end products(AGEs)and blood glucose,insulin,blood lipid and other related indexes was analyze.Spss25.0 statistical analysis software was used to process and analyze all data.Results: 1.17 patients in PCOS group,there are 2 people whose body mass index is less than25kg/m2,2 people between 25kg/m2~30kg/m2 and 13 people more than30kg/m2,12 patients in non PCOS groups,there are 3 people whose body mass index is less than25kg/m2,2 people between 25kg/m2~30kg/m2 and 7 people more than30kg/m2;2.There was not significantly different in age,weight,body mass index(BMI),waist circumference,hip circumference,waist to hip ratio between PCOS group and non PCOS group(P>0.05);3.The percentage of child born in PCOS group was significantly lower than that in non PCOS group(17.6% vs 58.3%,P<0.05);4.The testosterone and anti-Mullerian hormone in PCOS group were significantly higher than those in non PCOS group(0.71±0.255 vs 0.44±0.205,5.07±3.430 vs 1.30±0.785,P<0.05),and there was no significant difference between other sex hormones(P>0.05);5.The fasting insulin and homeostasis model assessment of insulin resistance(HOMA-IR)in PCOS group were significantly higher than those in non PCOS group(35.82±24.857 vs 14.48±9.560,7.83±4.643 vs 3.87±2.573,P<0.05);6.The areas under blood glucose curve in PCOS group were larger than those in non PCOS group,but there were no significance(12.18±0.761 vs 16.80±2.839,P>0.05),the areas under insulin curve and C-peptide(CP)curve in PCOS group were larger than those in non PCOS group(244.26±118.005 vs 109.43±80.073,13.72±3.834 vs 9.30±3.379,P<0.05),the curve of insulin,C-peptide(CP)showed a backward shift of secretion peak in non PCOS;7.The area,perimeter and length of omental adipocytes in PCOS groups were significantly larger than those in non PCOS group(5641.36±1769.614 vs4012.28±1971.602,297.22±50.581 vs 244.90±67.463,99.15±16.893 vs 81.95±21.929,P<0.05);8.In the patients whose body mass index is 25kg/m2,the percentage of cells with cell area ≥10000um2,cell perimeter ≥400um,and cell length ≥100um in PCOS group were significantly higher than those in non PCOS group(1.4% vs 0.0%,2.5% vs0.2%,21.3% vs 2.1%,P<0.05);In the patients whose body mass index between25kg/m2~30kg/m2,the percentage of cells with cell area ≥10000um2,cell perimeter ≥400um,and cell length ≥100um in PCOS group were significantly higher than those in non PCOS group(11.1% vs 1.1%,16.1% vs 1.1%,53.0% vs 7.2%,P<0.05);In the patients whose body mass index more than 30kg/m2,the percentage of cells with cell area ≥10000um2,cell perimeter ≥400um,and cell length ≥100um were no significant difference between PCOS group and non PCOS group(11.5% vs 8.6%,13.1% vs 9.6%,46.7% vs 43.8%,P>0.05);9.the difference of advanced glycation end products(AGEs)between PCOS group and non PCOS group was not significant(3700.07±1147.299 vs3887.19±1281.345,P>0.05);10.there was a positive correlation between advanced glycation end products(AGEs)and total cholesterol,low-density lipoprotein in PCOS group,r values are 0.5868,0.5578,P<0.05.Conclusion:1.There is hyperandrogenemia in PCOS group;2.Glucose metabolism in the PCOS group is mainly insulin resistance,while that in non PCOS group is mainly hyperglycemia;3.Although the abdominal circumference of both groups increased,the area,perimeter and diameter of omental fat cell in PCOS group are larger than those in non PCOS group.4.In PCOS group,the level of cholesterol and triglyceride increased,and cholesterol and low-density lipoprotein is positively correlated with advanced glycation end products.5.The number of cases in this study is small,and the sample size needs to be expanded for further study.