MiRNA Analysis Of Different Hormone Receptors In Endometrioid Adenocarcinoma

Objective: Study on the differential miRNA expression profile of different hormone receptor phenotypes in endometrioid adenocarcinoma,so as to find the specific target gene(miRNA-mRNA)of different subtypes of hormone receptors in endometrioid adenocarcinoma,to explore the possible regulatory mechanism of differentially expressed miRNA in different subtypes of hormone receptors in endometrioid adenocarcinoma,finally to provide theoretical guidance for molecular targeted therapy in the future.Methods: Collected postoperative specimens of endometrioid adenocarcinoma in NO.1 peoples Hospital of ChenZhou City from March 2019 to December 2019: 3 cases in ER(+)PR(+),estrogen receptor and progestogen receptor all positive、2 cases in ER(-)PR(-),estrogen receptor and progestogen receptor all negetive、2 cases in ER(+)PR(-),estrogen receptor positive and progestogen receptor negetive,and normal endometrium group is 3 cases.The chip hybridization experiment was carried out with American Agilent 21.0 version miRNA expression profile chip.The experimental data is conducted t-test.Screening criteria for miRNA is that the differential expression ratio equal 2 or higher than 2 and the p value lower than 0.05.Further more,screening for differentially expressed miRNA of three distinct subtypes of endometrioid adenocarcinoma.Target genes of differential miRNA were predicted and the target genes were analyzed by gene ontology and the signal enrichment pathway in GO database and KEGG database.Result: 1.The differential expression miRNA were 55、31、63 when the ER(+)PR(+)、 ER(-)PR(-)、 ER(+)PR(-)endometrioid adenocarcinoma compared with the normal endometrium,respectively,in each group the up-regulated miRNAs and the down-regulated miRNAs were 26、29;6、25;17、46;There were specially differential expression miRNAs 35 in the ER(+)PR(+)endometrioid adenocarcinoma,which up-regulated miRNAs 22 and down-regulated miRNAs 13.There were specially differential expression miRNAs 16 in the ER(-)PR(-)endometrioid adenocarcinoma,which up-regulated miRNAs 4 and down-regulated miRNAs 12.And there were specially differential expression miRNAs 45 in the ER(+)PR(-)endometrioid adenocarcinoma,which up-regulated miRNAs 11 and down-regulated miRNAs 34.2.The significant signal pathways of the ER(+)PR(+)endometrioid adenocarcinoma includes: MAPK 、 Phosphlipase D signaling pathway 、Pathways in cancer、PI3K-Akt、EGFR、et al.The significant signal pathways of the ER(-)PR(-)endometrioid adenocarcinoma includes: Apelin signaling pathway、Acute myeloid leukemia、Insulin signaling pathway、FOXO、Pathways in cancer,et al.The significant signal pathways of the ER(+)PR(-)endometrioid adenocarcinoma includes: Wnt、Signaling pathways regulating pluripotency of stem cell、MAPK、Gastric cancer、breast cancer、Pathways in cancer and so on.Conclusion: 1.There are specific differential expressed miRNA among different hormone receptor types in endometrioid adenocarcinoma,among them,the possible key miRNAs that the ER(+)PR(+)endometrioid adenocarcinoma are up-regulated miR-449a、miR-449b-5p、miR-182-5p、miR-96-5p、miR-200a-3p、miR-429、miR-141-5p,the ER(-)PR(-)endometrioid adenocarcinoma are up-regulated miR-20b-5p,down-regulated miR-124-3p,the ER(+)PR(-)endometrioid adenocarcinoma are up-regulated miR-5691,down-regulated miR-497-5p、miR-145-3p,the differential expression of these key miRNA is most likely to lead to different biological behaviors of different hormone receptors subtypes in endometrioid adenocarcinoma.2.There are different metabolic pathways in endometrioid adenocarcinoma with different hormone receptor subtypes which is preliminarily analyzed from the point of view of bioinformatics.Among them,the common signaling pathway of Pathways in cancer and PI3K-Akt may play an important role in the pathogenesis of endometrioid adenocarcinoma,while the specific signal pathways maybe related to the different biological behaviors of different hormone receptor types in endometrioid adenocarcinoma.