| Prostate cancer(PCa)is a common urogenital system tumor,which mostly occurs in elderly men and has a serious impact on men's physical and mental health.According to the us cancer data report of 2018,the incidence of prostate cancer among male malignant tumors occupies the first place.Compared with the low incidence of prostate cancer in China,the incidence of prostate cancer is gradually increasing as the aging trend continues to accelerate.At present,prostate specific antigen(PSA)is the main clinical test for prostate cancer.PSA is a specific protein with relatively high sensitivity and has become a common indicator for screening and diagnosis.However,PSA testing is influenced by a number of factors,leading to increased PSA in patients with benign prostatic hyperplasia.So it is difficult to judge the benign and malignant of prostate cancer.Heat shock protein(HSP)is involved in tumorgenesis,proliferation and other processes P.HSP family heat shock protein 90(HSP90)can be continuously detected in the peripheral blood of prostate cancer patients,and its level is related to the benign and malignant tumor and the presence of distant metastasis.HSP90 is a molecular chaperone that is involved in the stability of many client proteins,including androgen receptors and apoptosis suppressor proteins,making it an attractive molecular therapeutic target for prostate cancer.Therefore,it is of great research value and social significance to explore the relationship between HSP90 and the occurrence and development of prostate cancer,and to combine with other auxiliary indexes for the diagnosis,treatment and prognosis of prostate adenocarcinoma.Objective:To explore relationship between heat shock protein-90?(hsp-90?)and occurrence of prostate cancer,and clinical value of combined detection of serum hsp-90a and prostate specific antigen(PSA)in diagnosis of prostate cancer.Methods:A total of 30 patients with prostate cancer,30 patients with benign prostatic hyperplasia(BPH)and 30 healthy men(control group)were selected from September 2018 to September 2019,then to detect levels of serum hsp-90?,total PSA and free PSA(FPSA)by ELISA,serum testosterone level by radioimmunoassay,prostate cancer tissue was removed by operation,and relative expression of tissue hsp-90? protein by Western blot.Results:The levels of serum hsp-90? and total PSA in prostate cancer group were significantly higher than other two groups,and testosterone level was lower than other two groups(P<0.05);there was no difference of serum FPSA level between the three groups(P>0.05).It was found by Pearson test that serum hsp-90? was positively correlated with total PSA level,while negatively correlated with testosterone level(P<0.05).According to TNM stage of prostate cancer,there were 17 cases of stage ???,13 cases of stage ?-?,6 cases of Gleason score 1?,13 cases of 5?7,11 cases of 8?10,tumor diameter range from 0.8 to 6.2cm,with average of(3.9±1.5)cm.The relative expression of hsp-90? protein in tumor tissue was closely related to TNM stage,Gleason score and tumor diameter(P<0.05).By ROC analysis,it was found that accuracy of combined detection of serum hsp-90? and PSA levels for prostate cancer diagnosis was 0.896,and that of single PSA detection was 0.852.Conclusion:Higher expressions of hsp-90? in prostate cancer tissue and serum may be closely related to occurrence and development of prostate cancer,and combined detections of serum hsp-90? and PSA levels is of great significance in improving early diagnosis of prostate cancer. |
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