Clinical Application Of High-throughput Sequencing In HBV PreS Region Resistance Mutation And Genotyping

Objectives:Chronic infection with hepatitis B virus(HBV)has become a worldwide health problem,especially in China with a high prevalence of hepatitis B.With the long-term and widespread use of nucleoside drugs,the incidence of HBV resistance has become higher and higher,which has become a major problem in the clinical antiviral treatment process.This study used high-throughput sequencing,also known as Next Generation Sequencing(NGS),to perform deep sequencing of patients with chronic hepatitis B(CHB)to study NGS multi-drug resistance sites and gene scores in HBV.Type of application value.Methods:From May 2018 to March 2019,89 samples of HBV patients with HBV were collected from the First Affiliated Hospital of Kunming Medical University,the Second Affiliated Hospital of Kunming Medical University and the Kunming Infectious Disease Hospital.The HBV gene was detected by NGS.Type distribution and drug resistance locus analysis;improve the patient’s clinical data,analyze the differences in clinical laboratory indicators between wild type and mutant strains,and evaluate its clinical application value.Results:In the HBV genotyping test,33 cases of type B,52 cases of type C,and 4 cases of type B+C,the main study population was C type,accounting for 58.4%;9 cases were positive for mutant strain.A total of 7 drug-resistant mutation sites were detected:M/V207I,M204I,M250L,M250I,V173L,L180M,A180T,with the highest frequency of M/V207I single-base mutations(83.33%);9 positive mutant patients The most resistant sites were detected by 2071,followed by 2041;all were multi-drug resistant,the most common form of drug resistance was LAM+LdT;wild-type patients and mutant patients in liver function test indicators,HBV-There was no difference between the DNA copy number and the hepatitis B serum marker.Conclusions:High-throughput sequencing can provide quantitative results for HBV resistance genotypes and mutation sites,and visually analyze drug resistance;high-throughput sequencing can detect mutation sites when the mutation rate is<5%,and the mutation frequency is low.More mutation sites can be detected,which is of great significance for the discovery of potential drug resistance sites,has practical clinical application value,and can monitor the dynamic changes of potential drug resistance sites during the treatment of patients,which is conducive to the discovery and monitoring of new The form of drug resistance is particularly important for the realization of clinical individualized medications and the development of individualized treatment programs.