A Study On Differential Expression Profile Of MRNA And Long Non-coding RNA Based On Schistosoma Japonicum Schistosomula After Artesunate Treatment

Schistosomiasis,caused by schistosomes,is one of the parasitic diseases that seriously endangers human health,and is found in 75 countries and territories worldwide Approximately 600 million people are at risk of schistosomiasis infection,including nearly 300 million in Asia,Africa and South America.Although prevention and control of schistosomiasis are currently in place in endemic areas worldwide,the global incidence of schistosomiasis is still very high,with nearly 300 million people infected in Asia,Africa and South America.The incidence of the disease is still on the rise.Our country,which is an endemic area for schistosomiasis in Japan,is one of the four countries most severely affected by schistosomiasis on a global scale.In addition to the aggressive eradication of its intermediate host snails,pharmacological treatment remains the more effective approach in the control of schistosomiasis.Artesunate,a derivative of the antiparasitic drug artemisinin,has been shown to be effective in killing Japanese schistosomiasis child worms with Good placement of Japanese schistosomiasis,but the exact mechanism of artesunate against Japanese schistosomiasis has not yet been investigated.In recent years,with the development of bioinformatics and genome and transcriptome sequencing technologies,a large number of non-coding RNAs have been shown to be effective in numerous The artesunate plays an important regulatory role in biological processes.Therefore,it is important to search for the non-artesunate that is differentially expressed and has an important biological function in Japanese schistosomiasis child worms after artesunate action in the child worms.The coding RNA will lay a certain foundation for the elucidation of the mechanism of artesunate's action on Japanese schistosomiasis and the control of schistosomiasis.Provide new targets and ideasPURPOSEWe studied the differential expression profile of artesunate in the post-artesunate transcriptome of Schistosoma japonicum and analyzed the mechanism of artesunate regulation of the transcriptome of Schistosoma japonicum,which laid the foundation for further research on the molecular mechanism of artesunate action in Schistosoma japonicum and the discovery of new drug targets and future drug screening.METHODSThe larvae of Schistosoma japonicum obtained artificially from the larvae were cut off and cultured in vitro for 7 d.The larvae were separated from the larvae and cultured at 10 ?g/kg.mL and 20 ?g/mL of artesunate were used for in vitro culture of child worms,and the control group was added to the medium in the same volume.NaHCO3,and child worms were collected after 12 h of incubation for total RNA extraction.The Illumina Hiseq 6000 was used for the transcriptome after construction of a strand-specific library for rRNA removal Sequencing.After quality control of the sequencing results,the database is searched and analyzed using analytical software for RNA coding ability to screen for new possible The IncRN A and circRNA molecules were analyzed on the basis of bioinformatics to determine the differential expression level.The differentially expressed RNA molecules are combined with the results of GO and WEGG enrichment analysis to determine the potential biological functions of their target genes.Analysis.Finally,qPCR was used to detect some of the differentially expressed RNA molecules for validation.RESULTSA total of 16,550 expressed genes and 39,012 transcripts were obtained,which were screened based on the predicted coding ability of the transcripts.18259 mRNAs.according to differential expression analysis between the groups,comparing the experimental and control groups(S_ART-10/?20 Vs S_ART_C?A total of 3986 genes and 1274 mRNAs were significantly differentially expressed..Sequencing-derived transcript data were filtered to obtain a total of 6635 Novel lncRNAs,of which intergenic IncRNA accounted for 56.21%.According to the differential expression analysis between groups,it was found that comparing the experimental and control groups(S_ART-10/20 Vs S_ARTCA total of 358 lncRN As showed differential expression.lncRNA-mRNA targeting analysis showed that The number of mRNA-IncRNA pairs with homeopathic regulatory relationships is 18,of which 10 are positively correlated and 10 are negatively correlated.There were 8 pairs of circRNAs.According to the circRNA identification criteria,a total of 2495 circRNAs were obtained after screening,as well as 1603 The circRNA-hosting gene,in which more than 88%of circRNAs in each sequencing group are whole Exon-type circRNA.based on differential expression analysis between groups,comparing experimental and control groups(S_ART-?10/20 Vs S_ART_C?A total of 188 circRNAs showed significant differential expression.CONCLUSIONSThe non-coding RNA differential expression profile of artesunate on Schistosoma japonicum was studied for the first time by transcriptome sequencing technology,which laid the foundation for the molecular mechanism of artesunate on Schistosoma japonicum as well as drug target and new drug screening.