| Due to the increasing material needs of mankind,a large number of new compounds have been put into production and have been used in daily life.Most compounds have not been tested for toxicity when their risks are still unknown.Compounds may pose a threat to the ecological environment and human health,and such compounds are called environmental pollutants.How to quickly and accurately assess the toxicity of these environmental pollutants is a formidable challenge for toxicological research.Traditional toxicological experimental methods are based on animal in vivo experiments to evaluate toxicity,and such method is time consuming and labor intensive,expensive,ethical,and cannot avoid species differences.Therefore it is difficult to apply to the initial evaluation of a large number of environmental pollutants.The in vitro cell model can solve these problems to a certain extent,it can help to initially assess the harm of environmental pollutants to human health,and provide a preliminary basic theoretical basis for rationally guiding the use of environmental pollutants.Based on the two cell models of human mesenchymal stem cells,namely hMSC and Human adenocarcinoma alveolar basal epithelial cells,namely A549,this article separately evaluates the harm of two environmental pollutants,bisphenol compounds and nano titanium dioxide,to human cells.This article mainly includes the following three experiments:1.Bisphenol A,namely BPA,has a wide range of applications in daily necessities.Many literature have shown that BPA has developmental toxicity,which can disturb the metabolic homeostasis of human body and lead to a series of metabolic related diseases,including obesity and type II diabetes mellitus.This article proves that the exposure of low concentration of BPA can promote the proliferation of hMSC and promote its differentiation into adipocytes by using hMSC-induced adipogenic differentiation model,which provides a theoretical basis for further study on the toxic effect and mechanism of BPA and its substitutes.2.Due to the known toxicity,many countries have banned or restricted the production and overuse of BPA.It is extremely urgent to find structural analogs which can replace BPA.For now,the most commonly used alternatives are Bisphenol S(BPS),Bisphenol F(BPF)and Bisphenol AF(BPAF),but their health effects are still unclear.In this article,I use hMSC model to determine the basal toxicity of BPA,BPS,BPF and BPAF,and it is confirmed that BPS was more toxic to hMSC survival rate than BPA at low concentrations.In the mean time,at high concentrations,BPAF had higher damage to hMSC survival rate,cell cycle and mitochondrial membrane potential than BPA.Therefore,it is not a reasonable choice to use them to replace BPA for large-scale production and use.This experiment provides a preliminary basic theoretical basis for the further safety assessment of these four bisphenol compounds.3.Nano-TiO2 is widely used in cosmetics,functional fibers,plastics,paints,paints,etc.,because it can shield ultraviolet rays and it has good dispersibility and resistance.In the current chemical catalytic reaction experiments,to use reduced nano-TiO2 as a catalyst is an important development direction in the field of catalysis.However,in the process of development,generation and use,it is not clear whether nanoparticles will enter the human body with breathing,and whether it will affect the health of the human body.In this experiment,the A549 cell model was used to simulate the damage of human lung cells by inhaled nanoparticles in the process of production and use of oxidized nano-TiO2,reduced nano-TiO2 and nano-Ti02 with loaded platinum(Pt).The results demonstrated that these three nanomaterials did not affect the survival rate of A549 cells,but produced oxidative damage at high concentration.This provides more theoretical references for further exploring the toxicity of new nanomaterials and solving the safety problems of exposure(especially occupational exposure)during their research and development and production use. |
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