| Objective: To detect the effects of miR-141 on proliferation,apoptosis,invasion and migration of tongue squamous cell carcinoma(TSCC;Tscca,Tca-8113).Methods: Both groups of TSCC cells were stably transfected with miR-141 lentivirus Ubi-miR-141-SV40-EGFP-IRES-puromycin(miR-141 group)and lentiviral empty vector Ubi-MCS-SV40-EGFP-IRES-puromycin(miR-NC group);untreated cells were blank group(Blank).Reverse transcription quantitative polymerase chain reaction(rt-qPCR)was used to detect the expression of miR-141;flow cytometry was used to measure transfection efficiency and detect apoptosis.CCK-8 analysis,cell colony formation,wound-healing analysis,mirgration and invasion were performed to detect cell functions.The mRNA and protein expression of mmp2,mmp9,zeb2 and bcl-2 were detected by rt-qPCR and Western blotting.Result: The GFP transfection rate was determined by flow cytometry,in Tscca: was 82.4% in the miR-141 group and 87.3% in the miR-NC group,in Tca-811: was 73.4% in the miR-141 group and 89.1% in the miR-141 group 3.;rt-qPCR showed that,the expression of miR-141 was significantly higher than that of miR-NC group and Blank group,which proved that the stable transfected cell line was successfully established.There was no significant difference in cell proliferation between miR-NC group and Blank group(P>0.05).It indicated that lentiviral transfection had no effect on cell proliferation;however,it in miR-141 group was significantly lower than Blank group.The mRNA and protein expressions of mmp2,mmp9,zeb2 and bcl-2 were decreased in miR-141 group compared with miR-NC group and Blank group.Conclusion : In Tscca and Tca-8113 cells,overexpression of miR-141 inhibits cell proliferation and promotes apoptosis by regulating bcl-2 mRNA and protein expression;In addition,it may regulates mRNA and protein expression of mmp2,mmp9,Zeb2 to control cell invasion and migration.The mechanism maybe closely related to theepithelial-to-mesenchymal transition(EMT)process. |
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