Long-term Effect Of Clostridium Butyricum And Bifidobacterium On The Behavior Of Premature Mice

Objective: The aim of this study was to investigate the effect of clostridium butyricum and bifidobacterium on brain microglia activation and long-term behavior in premature mice.Methods: On the 16 th day of gestation,a preterm birth model was established by subcutaneous injection of mifepristone Capsule II(mifu RU486)into sterile pregnant mice(150ug/per mouse).The mice were randomly divided into three groups: preterm control group,preterm experimental group and term control group(10 in each group).The preterm control group and the preterm experimental group were born by the premature birth model of the pregnant mice,and the term control group were born by the natural birth of the pregnant mice without intervention.The preterm experimental group was given 10ul/g of Changlekang(clostridium Butyricum bifidobacterium bifidum live powder),the preterm control group and the term control group were given the same amount of normal saline at the same time.The weight of newborn mice was measured on P1,P8,P15 and P22.At the time of P30 to P34,the experiment of hidden platform of Morris water maze was carried out,and the experiment of space exploration of Morris water maze was carried out on P36.After the experiment of Morris water maze,the brain tissue samples of each group were taken,and the expression of ?-synaptophysin in brain tissue was detected by ELISA.Results:1.With the increase of days,the body weight of the three groups of mice increased(P<0.001);compared with preterm control group,the body weight of term control group was significantly higher on P1,P8,P15 and P22(P<0.05),however,therewas no significant difference in body weight between the preterm control group and the preterm experimental group on P1(P>0.05),but,on P8,P15 and P22,the body weight of the preterm experimental group was heavier than the preterm control group(P<0.05);compared with the term control group,the preterm experimental group had lower body weight on P1 and P8(P<0.001),but on P15 and P22,there was no significant difference between the two groups(P>0.05).2.With the increase of the number of days,the escape latency of the three groups of mice was decreased(P<0.05);on P30,there was no significant difference between the three groups(P>0.05),compared with the preterm control group,the escape latency of preterm experimental group and term control group was significantly shorter on P31,P32,P33 and P34(P<0.05),while there was no difference between the preterm experimental group and the term control group(P>0.05).3.Three groups of mice in the process of space exploration experiment,the number of times of crossing the platform and the ratio of staying time in the target quadrant were statistically significant(P<0.001);compared with the preterm control group,the number of times of crossing platform in the preterm experimental group and the term control group were significantly increased(P<0.001),the ratio of staying time in the target quadrant increased obviously(P<0.001),however,there was no significant difference in the two indexes between the preterm experimental group and the term control group(P>0.05).4.There was significant difference in the concentration of ?-Syn in the brain of three groups of newborn mice(P<0.05);compared with the preterm control group,the concentration of ?-Syn in the brain of the preterm experimental group and the term control group were significantly decreased(P<0.05),the concentration of ?-Syn in the preterm group tended to be normal(P>0.05).Conclusion:1.The growth and development ability of premature mice is low,long-term use of clostridium butyricum bifidobacterium can promote the growth and developmentof premature mice.2.Long-term feeding of clostridium butyricum bifidobacterium in premature mice can reduce the accumulation of ?-Syn in the brain,thus reducing the damage of microglia to neurons and improving the long-term behavioral development of premature mice.