Effects Of Sufentanil Combined With Cisplatin On Proliferation,Apoptosis,Migration,Invasion And Epithelial-Mesenchymal Transition Of Human Lung Cancer A549 Cells

OBJECTIVE To study the effects of different concentrations of sufentanil,alone or in combination with cisplatin,on the proliferation,apoptosis,migration,invasion and epithelial-mesenchymal transition of human lung cancer A549 cells,and to provide basis for perioperative analgesia and cancer pain treatment in tumor patients.Methods We cultured human non-small cell lung cancer A549 cells,and cultured A549 cells in RPMI-1640 medium containing 10%fetal bovine serum and 1%double antibody(penicillin mixed solution),placed at 37?%CO2 incubators.The cells were grouped into:control group,sufentanil(0.01,0.05,0.25 ?g/mL)group,cisplatin(5 ?g/mL)group,cisplatin(5?g/mL)+sufentanil(0.01,0.05,0.25?g/mL)group.CCK-8 method and plate colony formation experiment were used to detect the proliferation and cloning ability of A549 cells,Annexin V-FITC/PI double staining method was used to detect cell apoptosis,Transwell method was used to detect cell migration and invasion,and Western blot method was used to detect the following protein expressions:apoptosis Related proteins Bcl-2 and Bax,epithelial-mesenchymal transition-related proteins E-cad,N-cad and Vim,autophagy-related proteins p62,LC3.Results1.With the increase of the administration concentration,the CCK-8 experiment and the plate formation experiment showed that sufentanil enhanced the proliferation inhibition effect of A549 cells.Flow cytometry showed an increase in apoptotic cells.Transwell migration and invasion experiments showed that the number of through-mold cells was significantly reduced.Western blot results showed that compared with the control group,the expression of apoptosis-related protein Bcl-2 decreased,the expression of Bax increased,the expression of epithelial mesenchymal transition-related protein E-cad increased,and the expression of Vim and N-cad decreased.,The expression of autophagy-related proteins p62 and LC3-II increased;2.With the increase of the administration concentration,the CCK-8 experiment and the plate formation experiment showed that the sufentanil combined with cisplatin inhibited the proliferation of A549 cells.Flow cytometry showed an increase in apoptotic cells.Transwell migration and invasion experiments showed that the number of through-mold cells was significantly reduced.Western blot results showed that compared with the cisplatin group,the combined use of sufentanil and cisplatin decreased the expression of apoptosis-related protein Bcl-2,the expression of Bax increased,and the expression of epithelial-mesenchymal transition-related protein E-cad increased.The expression of N-cad decreased,and the expression of autophagy-related proteins p62 and LC3-II increased.CONCLUSION:1.Sufentanil can inhibit the proliferation,migration,invasion and epithelial-mesenchymal transition of human lung adenocarcinoma A549 cells,induce apoptosis,inhibit the autophagy of A549 cells,and are concentration-dependent;2.Sufentanil can increase the chemotherapeutic sensitivity of cisplatin and increase the inhibitory and killing capacity of cisplatin on human lung adenocarcinoma A549 cells.