Protective Effect Of Lactobacillus Rhamnosus GG On Acute Alcoholic Liver Injury

Background: Alcoholic liver disease(ALD)is a global disease.Due to the increasing morbidity and mortality year by year,it has received extensive attention.In our country,alcohol-induced liver injury has become a problem that cannot be ignored,and the prevention and treatment of alcoholic liver disease has become an important issue in China's medical field.Objective: To explore the protective effect and mechanism of probiotic bacteria Rhamnobacter GG(LGG)on acute liver injury in mice.Methods: C57 BL / 6 mice were used to divide the experimental mice into a control group,an alcohol group,an alcohol + LGG group,and LGG or MRS medium was continuously treated for 4 weeks with 50%(v / v)ethanol intragastrically to establish mice A model of acute liver injury.The content of ALT and AST in peripheral blood,liver coefficient,the content of glycerol(Glycerol),free fatty acid(FFA)and triglyceride(TG)in peripheral blood of each group of mice were studied.The liver TG content of each group of mice was detected and the histopathological changes were observed,and the expression of liver lipid metabolism related genes was also detected.By isolating mouse primary hepatocytes,in vitro experiments were used to further verify the regulatory effect of LGG on liver cell lipid metabolism.Results: Compared with the control group,the content of ALT and AST in the peripheral blood of mice in the alcohol group was significantly increased,but the content of ALT in the peripheral blood was significantly reduced after LGG pretreatment.Although the content of Glycerol,FFA and TG in the peripheral blood of mice increased after alcohol treatment and decreased after LGG pretreatment,it was not statistically significant.Although the liver coefficient did not change significantly between the mice in each group,compared with the control group,the liver TG content of the alcohol group was significantly increased and the liver TG content of the alcohol + LGG group was significantly reduced.At the same time,we stained liver tissue sections with H & E and Oil Red O,and observed similar results to liver TG content.In addition,the expression of fat synthesis-related genes in the liver tissue of mice in the alcohol group was significantly increased,and the expression of lipolysis-related genes was significantly reduced,while the expression of related genes in the alcohol + LGG group was reversed to varying degrees.It is worth noting that,compared with the control group,the phosphorylation level of AMPK in the liver tissue of mice in the alcohol group was significantly reduced,while the pretreatment with LGG significantly increased the phosphorylation level of AMPK.In in vitro experiments,we also found that alcohol-induced TG content in mouse primary hepatocytes was significantly increased and inhibited AMPK phosphorylation,while pretreatment with LGGs(LGG culture supernatant)significantly reduced cell TG content and promoted AMPK phosphorylation Change.Conclusion: R.rhamnosus GG can prevent and protect acute alcoholic liver injury in mice,and its protective effect is by regulating liver lipid metabolism.