Sphingosine Kinase 1(SPHK1) Participates In Brain Injury In The Early Stage Of Subarachnoid Hemorrhage(SAH) In Rats

Objective:to investigate the expression of Sphk1 in the early temporal lobe cortex after subarachnoid hemorrhage((SAH))and the role of(EBI)in early brain injury.Methods:the design of this study was divided into two parts.in the first part,the early autologous blood of adult male SD rats(250g-280g)was injected into the optic chiasmatic cistern to simulate subarachnoid hemorrhage model,3 h,6 h,12 h,24 h,48 h,72 h and 7 days after hemorrhage were established as time points.Westrenblot and immunofluorescence were used to detect the expression and specific localization of Sphkl.In the second part,the maximum time point of target protein expression was selected to further explore the effect of target protein on early brain injury after SAH.Overexpression plasmid and small interference reagent were injected into the ventricle to interfere with the expression of target protein.Westrenblot was used to verify the expression of target protein.Neurobehavioral tests and FJB staining were used to evaluate neurobehavior and apoptosis in rats.To explore the role of Sphkl in early brain injury after SAH.Results:1:through immunofluorescence and Westernblot test,we found that Sphkl increased gradually after SAH,reached the peak within 24 hours,and then decreased gradually.Second:neurobehavioral test we found that when the expression of Sphk1 in the brain increased,the neurobehavior of rats significantly improved,on the contrary,the performance of rats became worse.FJB staining showed that overexpression of Sphk1 could reduce neuronal apoptosis.It is suggested that Sphk1 is involved in the early brain protection of rats after SAH.Conclusion:We concluded that the expression of Sphk1 increased gradually after subarachnoid hemorrhage in rats,and gradually decreased to the level before modeling after reaching the peak.Up-regulation of Sphk1 can inhibit neuronal apoptosis and improve the neurobehavioral function of rats,so it can slow down the early brain injury after SAH and protect the brain.Therefore,increasing the expression of Sphk1 in rats is a potential strategy to improve SAH-induced EBI.