Hair Follicle Stem Cells And Bulb Dermal Papilla Cells In Vitro Culture Hair Follicle-like Organs

This study mainly describes the organ structure of the hair follicle,and the method for extracting hair papilla cells and hair follicle stem cells,as well asidentification markers and identification methods.The purpose of this study was to reconstruct hair follicle-like organs by co-cultivating hair follicle stem cells and hair papilla cells in vitro,and to provide a new treatment for hair loss treatment.In today's society,hair loss is becoming more common and younger.The problem of hair loss not only affects the appearance of patients,but also seriously affects the psychological state and quality of life of patients.Hair transplantation is the only long-term solution to the problem of hair loss.The essence of hair transplantation is the redistribution of self-advantaged resources.However,the current hair transplantation cannot solve the problem of insufficient hair in the donor area,such as FUE(Follicular Unit Extraction Technology).FUE refers to the technique of obtaining a single hair follicle unit graftdirectly from the donor area.That is,a ring drill is used to circumscribe a single hair follicle unit in the donor area,the connection between the hair follicle unit and surrounding tissue is cut off in the middle layer of the dermis,and then the hair follicle unit is completely removed.This method has the advantages of less trauma,no need for sutures,and quick healing after surgery,and is especially suitable for patients with scars or large scalp tension.However,FUE also has disadvantages:(1)the acquisition of hair follicle units takes a long time which is only suitable for the needs of hair transplantation in a small area;(2)During the operation,it is easy to damage the hair follicles and reduce the survival rate of transplanted hair.Studies have shown that hair follicle stem cells are mostly at rest with a slow periodicity in the body.It shows amazing proliferation ability in vitro or internal environment.Hair follicle stem cells have multi-directional differentiation potential.It can differentiate into epidermis,hair follicles,sebaceous glands,sweat glands,and other tissues.It can also directly inhibit the generation of scars through the regeneration of hair follicles during the process of skin wound healing.Hair papilla cells can still form hair papilla-like cell groups in vitro,which can provide a good basal environment for the growth of hair follicle stem cells.The multi-directional differentiation potential of hair follicle stem cells gives these two types of cells the ability to grow hair.Using homologous hair follicle stem cells and hair papilla cells to solve resistance problems and provide a new option for future hair transplants.Therefore,the use of hair stem cells in vitro culture,differentiation and expansion technology to obtain a sufficient number of autologous hair follicle units can theoretically solve this problem.And with the rapid development of tissue engineering and cloning technology,the selection and cloning of hair stem cells has become possible,which has laid the foundation for hair stem cell culture differentiation and expansion technology.By studying and summarizing various methods of culturing and screening hair follicle stem cells and hair papilla cells at home and abroad,the most suitable method for extracting and screening hair follicle stem cells and hair papilla cells is applied in this paper.First,culture and expansion in vitro were performed,cell suspensions were produced by mixing hair follicle stem cells and hair papilla cells and implanted into the back compartment of nude mice to observe the growing hair.Then,the hair was stained to observe the structure and function of the hair follicle-like organs.