Key Core Genes Of Hepatitis B Virus-Related Hepatocellular Carcinoma Based On Bioinformatics Approach

Objective:HBV-related HCC is the main type of primary liver cancer in China,and its morbidity and lethality rank first in the world,but the molecular mechanism of chronic HBV infection progressing to HCC is still not completely clear.The present study aims to identify Key genes related to HCC.Method:Through the comprehensive analysis of multiple gene expression from different profile data-sets,the differentially expressed genes between hepatocellular carcinoma and adjacent liver tissue samples were screened.Through the analysis of protein-protein interaction network,GO and KEGG enrichment and other analysis,the biomarker for the pathogenesis,diagnosis and prognosis of hepatocellular carcinoma were determined.Results:We identified 2 gene modules:containing 11 up-regulated key genes MCODE1 and 10 down-regulated key genes MCODE2,MCODE1 contains CCNB1,CCNB2,CDC20,MAD2L1,BUB1B,NUF2,ZWINT,BUB1,BIRC5,CENPF,CENPK.MCODE2 contains the following genes:CYP3A4,CYP1A2,CYP2E1,CYP2C8,CYP2C9,CYP2C19,CYP3A43,CYP26A1,CYP2B6,CYP39A1,these genes might be closely related to the pathogenesis of HCC,and CCNB1,CDC20,MAD2L1,ZWINT1,BIRC5,2 CYP1A2,CYP2C8,CYP2C9,CYP2C19 might be used as prognostic biomarkers for HCC patients.Gene expression differences between HCC tissues and adjacent tissues of CCNB1,CDC20,MAD2L1,CYP1A2,CYP2C8,CYP2C9,CYP2C19 were consistent with immunohistochemical characteristics,these genes might be expected to become a pathological marker of HCC.Conclusions:Through comprehensive bioinformatics research,it was found that the hepatocyte cycle,mitotic process and metabolic imbalance of various substances may play an important role in the occurrence and development of HBV related HCC.CCNB1,CDC20,MAD2L1,CYP1A2,CYP2C8,CYP2C9 and CYP2C19 are expected to become biological markers for the diagnosis,prognosis prediction and molecular targeted therapy of HCC.