Protective Effect Of Fecal Bacteria Transplantation(FMT)on D-galactosamine-induced Acute Liver Failure In Mice Based On Intestinal Flora

Objective:To investigate the protective effect of fecal bacteria transplantation(FMT)on D-galactosamine-induced acute liver failure in mice,and to explore its mechanism through experiments and principles.Methods:85 male BALB/c mice were randomly divided into 6 groups:a normal control group of 10(CON):standard feed feeding without special treatment;b model group of 15(DG):intraperitoneal injection 2 weeks after feeding D-aminogalactose;c FMT treatment group:15 animals:intraperitoneal injection of D-aminogalactose 2 weeks after feeding;after successful injection,FMT bacterial solution was given intragastrically(200ul/head)for three days;d FMT prevention 14 days group 15 animals:FMT bacterial solution was given intragastrically for 2 weeks(200u/piece)at the time of feeding,and D-aminogalactose was intraperitoneally injected on the second weekend;e FMT prevention 7 days group 15 animals:FMT bacterial solution was given intragastrically for 1 week at the feeding(200ul/head),intraperitoneal injection of D-galactosamine on the second weekend;f FMT prevention 3 days group of 15:FMT bacterial solution was given intragastrically for 3 days(200ul/head),and D was injected intraperitoneally on the second weekend-Galactosamine.All 6 groups were fed with standard feeds.The injection amount was 3.0 g/kg of D-galactosamine(dissolved in physiological saline at a concentration of 60 mg/ml)to control variables.The feces of the test subjects were collected at the beginning and end of each group and before and after treatment with FMT,and mouse feces DNA was extracted using a kit for 16s rRNA sequencing.Subsequently,the serum,plasma and liver tissues of each group of subjects were collected for detection and analysis.The specific testing items are as follows:the serum ALT and AST levels are measured by an automatic biochemical analyzer;the serum IL-10 and IL-6 levels are measured by an enzyme-linked immunosorbent assay(Elisa);the kit is used to check the plasma LBP levels;Partial liver tissue was fixed with 10%neutral formaldehyde solution,and then stained with conventional hematoxylin-eosin-(HE).After successful staining,the pathological changes were observed in an electron microscope.The effect of FMT on acute liver failure in mice was evaluated by the results of survival rate,phenotypic observation,changes of serum inflammatory factors and intestinal flora.Results:The survival rate of the model group was significantly lower than that of the prevention group and the treatment group;the body weight and liver weight of the mice in the FMT 14-day prevention group,the FMT 7-day prevention group and the treatment group were significantly increased compared with the model group(p<0.05);the FMT 14-day prevention group,FMT7 The pathological damage of liver tissue in mice in the Tian prevention group and the treatment group was lighter than that in the model group;the serum anti-inflammatory factor IL-10 in the mice of the FMT 14 day prevention group,the FMT 7 day prevention group and the treatment group was significantly higher than the model group(p<0.05).),And the pro-inflammatory factors IL-6 and LBP were lower than those in the model group(p<0.05);the diversity of intestinal flora of mice in the FMT 14-day prevention group,FMT 7-day prevention group and treatment group was higher than that in the model group,and probiotics were abundant.Degree increased significantly.Conclusion:FMT significantly improved the survival of D-aminogalactose-induced ALF mice and alleviated their acute liver failure,and the specific mechanism of action may be related to the altered intestinal flora structure.