Effect Of Silencing FOXC1 Gene On The Proliferation And Apoptosis Of Glioma Cells Regulatd By Notch Pathway

Objective Glioma is one of the neurological diseases that seriously threaten human survival.It has a high incidence rate and a high degree of malignancy.In recent years,gene therapy has been gradually recognized and widely studied,but the specific target and mechanism of action have not been determined.This study investigated the effect of silencing FOXC1 gene on the proliferation and apoptosis of glioma cells regulatd by Notch pathwayMethods According to the earlier experiments,siRNA specifically targeting FOXC1 gene(FOXC1-siRNA-1707)and the negative control sequence(FOXC1-siRNA-NC)were selected,and then were transfected into two kinds of human glioma cells(glioma U251 cells and glioma TJ905 cells)by Lipofectamine2000 DNA Transfection Reagent.The MTT assay was used to detect cell proliferation changes at different time points(6h,12h,24 h and 48 h).Flow cytometry analysis was used to detect apoptosis rate(Represented by subapoptosis peak)of glioma cells.Western blot was used to detect Changes in Notch-1 and DLL4 protein levelsResults MTT assay showed that in the glioma U251 cells,the proliferation rate of the experimental group(FOXC1-siRNA-1707 group)was decreased compared with that of the control group and the negative control group(FOXC1-siRNA-NC group),and it's time dependent,the difference was statistically significant(Ftreatment group=80169.78,Ftime=115031.3,Ftreatment group×time=5604.137,all P<0.05);Compared with the control group,the cell proliferation rate of the negative control group(FOXC1-siRNA-NC group)decreased,but the difference was not statistically significant(P>0.05).In TJ905 glioma cells,the proliferation rate of cells in the experimental group(FOXC1-siRNA-1707 group)was lower than that in the control group and the negative control group(FOXC1-siRNA-NC group),and it's time dependent,the difference was statistically significant(Ftreatment group=111037.1,Ftime=153277.7,Ftreatment group×time=5208.2256,all P<0.05).Compared with the control group,the cell proliferation rate of the negative control group(FOXC1-siRNA-NC group)decreased,but the difference was not statistically significant(P>0.05).The results of flow cytometry showed that in U251 cells,the apoptosis rate of glioma cells increased after the silencing of FOXC1 gene,and the difference was statistically significant(F=155.9,P<0.01).And in TJ905 cells,the apoptosis rate of glioma cells also increased after the silencing of FOXC1 gene,and the difference was statistically significant(F=243.1,P<0.01).Western blot results showed that in U251 cells,after targeted silencing of FOXC1 gene,the expression levels of Notch 1 and DLL4 protein decreased,with statistically significant differences(F values were 7.408 and 12.305,respectively,P<0.05).And in TJ905 cells,after targeted silencing of FOXC1 gene,the expression levels of Notch 1 and DLL4 protein also decreased,with statistically significant differences(F values were 8.742 and 19.204,respectively,P<0.05).Conclusions Target silencing FOXC1 gene can inhibit the proliferation of glioma cells and promote apoptosis.The mechanism may be related to the regulation of Notch pathway.