The Inhibitory Effect Of LncRNA AC006050.3 Knockdown On Proliferation,Migaration,Invasion Of Gastric Cancer Cell BGC-823

Background and Objective:Gastric cancer is one of the most common malignant tumors with a high incidence rate and high mortality rate in the world.It seriously affects human health and living quality.Proliferation,migration and invasion of gastric cancer cells are important factors affecting the development and prognosis of gastric cancer.Long non-coding RNA(lncRNA)is a kind of RNA without coding function with length more than 200 nt.It plays important roles in many biological process,such as regulating transcription,epigenetics.lncRNA AC006050.3 locates at human chromosome 17 with a length of 160585 bp.It was found mainly in advanced biology.There are no clear conclusions about the relationship between AC006050.3 and digestive system tumors.On the bases of previous fundings,we found that AC006050.3 highly express in gastric cancer cells and relate with migration,proliferation and invasion of BGC-823 cells.So we will detect the possibility of using AC006050.3 as a molecular target for the diagnosis and treatment of gastric cancer patients.Method:(1)Using qRT-PCR confirms the expression of AC006050.3 in gastric cancer cell lines(MKN-45,SGC-7901,BGC-823)and GES-1.(2)Transfecting siRNA into BGC-823,and verifying transfection efficiency by qRT-PCR.(3)CCK8 assay,wound healing assay,transwell assay were used to detect the correlation between AC006050.3-si and proliferation,invasion and migration.(4)The expression level of MMP-2 and MMP-9 after silencing AC006050.3 in BGC-823 were compared by western blotting.Results:(1)AC006050.3 highly express in gastric cancer cell lines(MKN-45,SGC-7901,BGC-823)(P < 0.05).(2)The expression level of AC006050.3 in BGC-823 was significantly decreased after transfecting the silencing the AC006050.3(P < 0.001).(3)CCK8 results showed that the proliferation ability of BGC-823 was weakened after the AC006050.3 was down-regulated(P < 0.05).Wound healing result shows that the width of the wound is longer in the positive group than the negative control group(P < 0.05).Transwell assay results showed that not only migration ability,but also invasion ability decreased after the AC006050.3 was down-regulated(P < 0.001).(4)The expression level of MMP-2 and MMP-9 decreased after AC006050.3was down-regulated(P < 0.05).Conclusion:(1)The expression of AC006050.3 increased in gastric cancer cells.(2)Inhibition of AC006050.3 may inhibit the proliferation,migration and invasion of BGC-823.(3)AC006050.3 is expected to be a new molecular target for targeted treatment of gastric cancer.