MiR-1224-3p Regulates Aerobic Glycolysis And Proliferation And Migration Of Breast Cancer Cells By Targeting PGM5

Phosphoglucomutase-like protein 5(PGM5)is a member of the PGM family,which plays an important role ni glucose metabolism and glycogen metabolism by catalyzing bidirectional conversion of grape-1-phosphate(G-1-P)and glucose-6 of-phosphate(G-6-P)Aerobic glycoly sis,which tumor cells rely primarily on for energy even under conditions of adequate oxygen supply,makes tumor cells highly glycolytic and requires an increased need for glucose.However,the role of PGM5 in aerobic glycolytic pathways,as well as its effect on breast cancer development and progression is still unclear.With bioinformatics analysis,we found that PGM5 expression is downregualted in breast cancer tissues compared to para-cancerous tissues,nd high expression of PGM5 is positively correlated with good prognosis of breast cancer patients,suggesting that PGM5 may play a role in breast carcinogenesis and cancer progression.Using cell proliferation experiments and wound healing experiments,we found that overexpression of PGM5 inhibits proliferation and migration of breast cancer cells,whereas knockdown of PGM5 with small interfering RNA promotes breast cancer cell proliferation and migration,Western blot analysis demonstrated that PGM5 regulates the expression of cell cycle-and apoptosis-related genes.Combining bioinformatics prediction with microRNA transfection and dual luciferase reporter analysis,we found that miR-1224-3p binds to PGM5 3’-UTR(untranslated region)and inhibits the expression of PGM5.Further experiments showed that miR-1224-3p/PGMS axis modulates breast cancer cells proliferation and migration as well as regulation aerobic glycolysis.In summary,miR-1224-3p/PGMS axis links aerobie glycolysis with breast cancer cells and is potential targets for the diagnosis,treatment and prognosis of breast cancer.