Expression Of Immune Factors In Peripheral Blood And Middle Ear Effusion In Children With Otitis Media With Effusion With Or Without Allergic Rhinitis

Objective:The etiology and pathogenesis of otitis media with effusion(OME)are complex,and it is not yet clear.In recent years,there have been many researches on the immunity of secretory otitis media in adults,but few in children.The purpose of this study is to investigate whether there is a difference in Th2 cytokine profiles in peripheral blood and middle ear effusion in children with secretory otitis media with or without allergic rhinitis,and an in-depth understanding of the role of allergies in the formation of OME can help better To understand the pathogenesis of OME,to provide theoretical guidance for the treatment and prevention of OME.Methods:Thirty patients aged 3-14 years who were diagnosed with chronic OME and underwent tympanotomy intubation in our hospital were selected.13 patients with secretory otitis media and allergic rhinitis were selected as the experimental group,and17 patients with secretory otitis media were not allergic.Rhinitis was used as the control group.Samples and serum samples of middle ear effusion were obtained from patients and the concentrations of IL-6 and IL-10 in peripheral blood and middle ear effusion were evaluated by enzyme-linked immunosorbent assay(ELISA).Resultts:The concentration of IL-6 in the peripheral blood of the experimental group was higher than that of the control group,and the difference was statistically significant(P<0.05);the concentration of IL-10 in the peripheral blood of the experimental group was not significantly different from that of the control group(P> 0.05)(Table 1).The concentration of IL-6 in the middle ear effusion in the experimental group was higher than that in the control group,and the difference was statistically significant(P <0.05);the concentration of IL-10 in the middle ear effusion in the experimental group was not significantly different from the control group(P> 0.05)(Table 2).The concentration of IL-6 in the middle ear effusion in the experimental group was higher than that in peripheral blood,and the difference was statistically significant(P <0.05);the concentration of IL-10 in the middle ear effusion in the experimental group was lower than that in peripheral blood,and the difference was statistically significant(P <0.05)(Table 3).The IL-6 concentration in the middle ear effusion of the control group was higher than that of peripheral blood,and the difference was statistically significant(P<0.05);the IL-10 concentration in the middle ear effusion of the control group was lower than that of peripheral blood,and the difference was statistically significant(P <0.05)(Table 4).Conclusions: The occurrence and development of OME is related to the allergic mechanism.Allergic rhinitis promotes and exacerbates OME,and is also one of the main causes of refractory and recurrent OME.IL-6 and IL-10 are two important immunoregulatory mediators,and their changes lead to the imbalance of TH-1 / TH-2,which leads to the occurrence of immune diseases.The middle ear cavity may be a target organ for allergic inflammation,suggesting that proper allergy management may be a useful adjunct to OME management.Therefore,it is feasible to block the occurrence and development of OME by changing the concentrations of IL-6 and IL-10 to treat the occurrence of allergic diseases.