Study On The Mechanism Of Atractylodes Macrocephla Polysaccharides Regulating Inteinal Microflora And Sensory Ability Of Tail-suspended Rats

The problem of astronauts suffering from infectious diseases in the aerospace weightlessness environment is gradually revealed in front of people.A series of physiological and pathological changes that occur in various systems of the body in a weightless environment,such as changes in microbial traits and pathogenicity,pose major challenges to aerospace medical research and implementation.Weightlessness can have a certain effect on the gastrointestinal mucosal barrier function.The intestinal flora in the human body is closely related to the intestine.The rich intestinal neural network and brain-gut axis system in the intestine cause changes in the intestinal environment to affect various tissues of the body.In a recent study,it was found that the abundance of intestinal flora will change under the space environment.However,there is no in-depth research at present.Some studies have found that Atractylodes macrocephala polysaccharides can regulate the intestinal flora steady state destruction response.The treatment has good effects.Therefore,Atractylodes macrocephala polysaccharides may have the potential to regulate intestinal flora under simulated microgravity.As a preventive and therapeutic agent.The experimental materials used in this paper are Wistar rats.The tail-hanging method was used to establish a simulated microgravity model.After 28 days of cultivation,they were divided into five groups.That is,the ground group,tail suspension group,low,medium and high dose group.The experiment collected 28-day rat feces from different groups,extracted six representative bacterial DNA from the feces,and applied real-time quantitative PCR technology to determine their genomic DNA.The results found that:Simulated microgravity can lead to an imbalance in the steady state of the intestinal microenvironment.It is shown that the number of probiotics of Bacteroides,Lactobacillus,Enterococcus,and Bifidobacterium is significantly reduced compared with the ground control group;Clostridium,E.coli The number of pathogenic bacteria increased significantly.In the low-,medium-,and high-dose gavage Atractylodes macrocephala polysaccharide group,it was found that the number of probiotics increased significantly and the number of pathogenic bacteria decreased significantly compared with the tail-suspended group.The significance of the dose group varies according to different microflora.On the 28 th day,the pain sensation ability of rats was measured with a hot stinging instrument respectively,based on the time when the hindlimbs of the rats were lifted after sensing the pain sensation;the motor coordination ability was measured with the rat treadmill,and the rat fell on the electrical stimulation track area Based on the number of times.The results found that simulated microgravity can cause the ability to perceive pain and exercise coordination to be affected.Compared with the ground control group,the sensitivity to pain perception and the ability to coordinate movement were significantly reduced.In the low-,medium-,and high-dose infusion group of Atractylodes macrocephala polysaccharides,it was found that compared with the tail-suspended group,there was a significant recovery of the ability to perceive pain and exercise coordination.The significant performance of the dose group varies.And according to the results of the previous laboratory,through the analysis of the Biogps database information,it was found that the neuropeptide Y,substance P,vasoactive intestinal peptide are closely related to the perception ability.It provides an important theoretical basis for the future research on the specific mechanism of Atractylodes macrocephala polysaccharides regulating tail adjustment on perception ability.In summary,the gut microbiota became disordered after simulated weightlessness.The sensory perception of the body changes,and the motor coordination ability is abnormal.Atractylodes macrocephala polysaccharides can alleviate the effects of intestinal flora and body perception and exercise convenience.It may be used as a protective agent against physical health damage when astronauts perform space operations in the future.