| Cerebral ischemic disease is a common acute cerebrovascular disease,characterized by high recurrence and high death,so that its treatment is the focus of research.Salvia miltiorrhiza is a classic drug for promoting blood circulation and removing blood stasis,Dalbergia odorifera also has the effect of removing blood stasis and hemostasis.The combination of these two traditional chinese medicines is often used to treat cardiovascular and cerebrovascular diseases.In this paper,Danshen injection and Xiangdan injection were used as the representatives,and the material basis study of Salvia miltiorrhiza-Dalbergia odorifera on anti-cerebral ischemia was carried out from the perspective of metebolism.The specific contents include the following aspects: 1.The contents of the effective components in salvia miltiorrhiza and Dalbergia odorifera were determined according to the provisions of the Chinese Pharmacopoeia,a comprehensive HPLC method was established to determine the contents of danshensu,protocatechuic aldehyde,protocatechuic acid,caffeic acid,p-coumaric acid,salvianolic acid A,rosmarinic acid,cryptotanshinone,tanshinone IIA,vanillin and vanillic acid in injections.The results showed that the total contents of cryptotanshinone,tanshinone IIA and tanshinone I in Salvia miltiorrhiza was greater than 0.25%,the contents of Salvianolic acid B was greater than 3.0% and volatile oil in Dalbergia odorifera was more than 1.0%.The contents of protocatechuic aldehyde in the injections were higher than the required standard content.It indicates that the medicinal herbs and the injections were in compliance with the pharmacopoeia and the prescription of traditional Chinese medicine and the quality was qualified,which could be used in subsequent experiments.2.Using HPLC-Q/TOF-MS metabolomics research method combined with pharmacodynamics research,to complete metabolomics research of the Salvia miltiorrhiza-Dalbergia odorifera on Anti-cerebral ischemic.The results showed that the levels of 20 endogenous small molecules in the model group had disordered compared with the sham operation group.Both Danshen injection and Xiangdan injection could improve these compounds to different degrees.The high-dose group of Xiangdan injection had the most obvious effect.The related metabolic pathways includes: Amino acid metabolism,Glutathione metabolism,sphingomyelin metabolism,fatty acid metabolism,etc.At the same time,the drug component: caffeic acid and the metabolites: IDHP and KYBZ were found in the high-dose group of Xiangdan,which provided a basis for the follow-up exploration of the ingredients absorbed into the brain of Salvia miltiorrhiza-Dalbergia odorifera.3.Using the MD-HPLC-ECD technology to study the effect of Salvia miltiorrhizaDalbergia odorifera on neurotransmitters of rats with cerebral ischemia and metabolism in the brain.The results showed that the neurotransmitters dopa,tyrosine,unkown compounds M1 and M2 in the MCAO group significantly changed compared with the sham operation group.After administration,Xiangdan injection could significantly regulate the level of disordered neurotransmitters and the effect was better than Danshen injection.At the same time,it was found that the Xiangdan injection group had more ingredients that could enter the brain than that in the Danshen injection group.It illustrated that the combination of Salvia miltiorrhiza and Dalbergia odorifera can promote the drug ingredients such as: danshensu,rosmarinic acid,salvianolic acid A,and metabolites: IDHP and KYBZ into the brain,and speculated that these components may be related to the abnormal changes of neurotransmitters in the brain regulated by Xiangdan injection. |
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