The Research Of Methods In Rapid Vascularized Organization Based On A-G-IPN Hydrogels

Objective: Vascular system research is of vital important for manufacturing suitable tumor models and the development of effective clinical therapies.With progresses on biomedicine,computer science,mechanical engineering and other fields,Stereolithography Apparatus technology,Fused Deposition Modeling technology,Three-dimension Printing technology and other advanced molding technology have been developed for vascular system model construction.However,the construction of vascular system in vitro is still in initial,and some problems,such as poor in mechanical properties,connectivity and processability,should be improved.The aim of this study was to design methods for rapid construction of vascular network precursors in vitro and improve their mechanical properties,connectivity and processability.Methods: This paper used sodium alginate/gelatin as matrix materials to prepare a double crosslinking interpenetrating network(IPN)hydrogel with a certain mechanical strength and biological performance through a series of structural and biological tests.Based on the material and 3D printer,several methods to quickly build vascular network precursor were designed: bubbles,single nozzle printing with injecting,single nozzle immersive printing and double nozzle printing.The result analysis of structural and biological tests was arranged to evaluate the methods.Results: 1.The cells throve in A-G-IPN hydrogel and could attached to the infiltrating scaffold for cell metabolism,proliferation and other activities.2.The three-dimensional scaffold model with connected multi-channel could be construct by 4 methods: bubbles,single nozzle printing with injecting,single nozzle immersive printing and double nozzle printing.3.The ADSCs in three-dimensional scaffold model were differentiated into ECs,and 4 methods mentioned above could be applied to construct the vascular network precursor.Conclusion: The A-G-IPN hydrogel can be used as matrix materials to construct a 3D cell culture system for cell metabolism,proliferation,differentiation and other activities.The vascular network precursor can be rapidly constructed by 4 methods: bubbles,single nozzle printing with injecting,single nozzle immersive printing and double nozzle printing.