| Objectives By detecting the glycolipid profile and the structural characteristics of intestinal microflora of pregnant women with reasonable and excessive weight gain during pregnancy,the differences of intestinal microflora and lipid profile between the two groups were compared,and the effects of too fast weight gain on intestinal microflora and lipid profile of pregnant women were discussed.At the same time,the effects of too fast weight gain on neonatal outcome were analyzed,so as to study the effects of excessive weight gain during pregnancy on maternal and infant health and perinatal health The influence of labor outcome provides theoretical basis.Methods From January to June 2019,17 pregnant women with normal weight gain and 22 women with excess weight gain were selected and delivered in the Affiliated Hospital of North China University of technology.The fecal samples of the subjects were collected before delivery.The 16 Sr DNA gene v3v4 variable region was used for miseq pe300 double terminal sequencing to analyze the normal group and the super recombinant intestinal flora.The diversity and abundance of the intestinal flora of the two groups were analyzed by bioinformatics.At the same time,3-5ml of pregnant women ’s fasting venous blood was taken and sent to the laboratory of our hospital within 20 minutes to detect the levels of TG,TC,LDL-C,HDL-C and glycosylated hemoglobin in the mother blood and cord blood.Organize the data for statistical analysis.Results 1.Comparison of general basic data of two groups of pregnant women: the prenatal BMI of super recombined pregnant women was higher than that of normal pregnant women,with statistical significance(P < 0.05);other indicators(age,pregnancy,delivery,BMI before pregnancy,education level,eating times)were not statistically significant(P > 0.05),and the two groups were comparable;2.Comparison of blood glucose and lipids: the Tg of super recombined maternal blood was higher than that of the control group(6.04 ± 2.76v3)The difference was statistically significant(t =-2.835,-3.134,P < 0.05).The Hb A1 c level of the super recombined pregnant women was higher than that of the normal group(5.15 ± 0.74v4.45 ± 0.46)mmol / ml,the difference was statistically significant(t =-3.407,P < 0.05)Factors(Wald χ 2 = 5.744,P < 0.05);3.The maternal and infant outcomes of the two groups: postpartum hemorrhage was different,the super recombination was greater than that of the normal group(140.46 ± 60.35v104.71 ± 29.18)ml,the difference was statistically significant(t =-2.434,P = 0.021),and other indicators(neonatal weight,neonatal bilirubin,neonatal Apgar score,prom,amniotic fluid pollution,delivery mode)had no significant difference.4.Sequence and structure comparison of intestinal flora in the two groups showed that there were 10 faeces in the group of excessive weight gain during pregnancy,which were phylum Firmicutes(71.18%),Bacteroidetes(11.64%),actinobacteria(8.04%),Proteobacteria(4.89%),Euryarchaeota(3.45%),verrucomicrobia(0.50%),cyanobacteria(0.50%)Cyanobacteria(0.09%),Candidatus sacchari bacteria(0.07%),fusobacteria(0.03%),tenerictes(0.01%),others(0.10%).92 genera were found at the genus level,among which 4 genera were found with relative abundance greater than 5%.They are: Bacteroides(12.25%),blautia(7.69%),Bifidobacterium(6.27%),Bacteroides(7.32%).There were 10 fecal samples in the group with reasonable weight gain during pregnancy,which were: posterior mycotes(70.48%),Bacteroidetes(13.58%),actinobacteria(8.55%),Proteobacteria(5,50%),Euryarchaeota(0.80%),verrucomicrobia(0.87%),cyanobacteria(0.05%),candidat(0.05%)92 genera were found at genus level,including 4 genera with relative abundance greater than 5%.They are: Bacteroides(14.52%),BL autia(9.55%),Bifidobacterium(6.66%),Prevotella(7.10%).The alpha diversity of two groups of samples(Chao1,observed_There was no significant difference in the species,Shannon,Simpson and beta diversity(PCo A,NMDS).There were differences in rank sum test between groups: the abundance of Streptococcus and Gardnerella was higher than that of lachnospiracea in normal group_incertae_Sedis, anaerotruncus and weissella have higher abundance.Conclusions 1.The level of TG and Hb A1 c in the maternal blood of the over increased pregnant women was significantly higher than that of the moderate weight gain group,and HDL-C was significantly lower than that of the moderate weight gain group;the level of TG in the maternal blood of the over increased pregnant women was related to the over weight gain;2.The level of blood glucose in the maternal blood of the over increased pregnant women was significantly higher than that of the moderate weight gain group;3.The level of Streptococcus,gard The abundance of Gardnerella was higher than that of lachnospiracea_incertae_There was no significant difference in the structure and diversity of intestinal microflora in pregnant women with simple weight gain but no other complications.Figure18;Table11;Reference 150... |
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