| Objective : 1.To learn and master the technique of hyperplasia scar modeling in SD rats.2.To explore the optimal concentration of pentoxifylline for the intervention of hypertrophic scars in SD rats.3.To study the effect of pentoxifylline on TGF-β1 /Smad signal transduction pathway in hypertrophic scars of SD rats.Methods: 1.Modeling by scalding,SD rats are fixed,anesthetized,skin prepared,disinfected,and heated with a homemade copper sheet on a hot plate,acting on a square area with a length of 2 cm on both sides of the back of the rat,waiting for its natural growth After 28 days,tissues were taken for pathological confirmation.2.Divide the successfully modeled SD rats into 4 groups,3 rats in each group,4 scar tissues,and respectively inject 0.5g / L,1.0g / L,1.5g / L,2.0 into the scar g / L pentoxifylline solution,once a week for a total of 6 weeks,then take scar tissue pathological sections for HE staining,and count fibroblasts under a general optical microscope(HE × 400),each section is randomized per person Three fields of vision were taken and counted separately.A total of 3 persons were repeated.As a result,the average of 3 persons was used to detect the optimal concentration of pentoxifylline.3.Divide the rats into 2 groups according to whether they are moisturizing with allantoin cream externally,moisturizing group and non-moisturizing group,each group is divided into four groups:(1)experimental group(the most suitable concentration group of pentoxifylline);(2)positive control group(Quannide group);(3)negative control group(distilled water group);(4)blank control group.In addition to the blank control group,the other groups received corresponding interventions in the scars once a week for 6 weeks.Take HE staining of the pathological tissue every 2weeks,count the fibroblasts,and detect the proliferation of scar fibroblasts;after 6weeks,surgically remove the scar tissue of each group,and detect TGF-β1,Smad2 / 3and P-smad2 / 3 by Western blot 3.Expression.4.Perform statistical analysis on the experimental results.Results: 1.After 6 weeks of administration,the number of fibroblasts in the 2.0g / L pentoxifylline solution group was less than that of other concentration groups,and the differences were statistically significant(P <0.05),2.0g / L was the self The optimal concentration of ketoxifylline for inhibiting the proliferation of hypertrophic scar fibroblasts in SD rats.2.Pentoxifylline can inhibit the proliferation of scar fibroblasts in the moisturizing and non-moisturizing groups.In the non-moisturizing group,the number of experimental fibroblasts in the non-moisturizing group was less than that of the positive control group,but the difference was not statistically significant(P>0.05),4 and 6 weeks after administration,the number of fibroblasts in the experiment was less than that of the positive control group,negative control group and blank control group,the difference was statistically significant(P <0.05).4.Compared with the corresponding groups between the moisturizing group and the non-moisturizing group,the expressions of TGF-β1,Smad2 / 3 and P-smad2 / 3 were not significantly different(P> 0.05).There was no statistically significant difference in the expression of TGF-β1,Smad2 / 3 and P-smad2 / 3 between the moisturizing group and the non-moisturizing group.(P> 0.05).Conclusions: 1.Successful establishment of SD rat hypertrophic scar model.2.Pentoxifylline can effectively treat hypertrophic scars of SD rats,the optimal concentration is 2.0g / L.3.Local injection of pentoxifylline can inhibit the proliferation of hypertrophic scar fibroblasts.4.The effect of pentoxifylline in inhibiting hypertrophic scars may not be through the TGF-β1 / Smad signal transduction pathway. |
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