PD-1 Nucleotide Polymorphism And The Risk Of Hepatitis B Infection

Purpose: To explore the relationship between PD-1 single nucleotide polymorphism and hepatitis B infection.Methods: The single nucleotide polymorphisms of rs10204525,rs2227982,rs41386349 and rs36084323 of PD-1 genes were detected by Taqman probe on the DNA extracted from the peripheral blood of 222 asymptomatic Carrier,276 CHB patients,295HBV-related liver cirrhosis,and 105 acute-on-chronic liver failure of hepatitis B.Results: PD-1 gene rs10204525,rs2227982,rs41386349 and rs36084323 all conform to hardy-weinberg genetic equilibrium law after testing.For allele frequency analysis,G allele of rs10204525 was protective factor(OR=0.823,95%CI=0.679–0.997,P=0.046)and T allele of rs2227982 was predisposing factor(OR=1.231,95% CI=1.036–1.463,P=.018)in HBV infection.When analyzed in genotype frequency,the genotype GG of rs10204525 and CC of rs2227982 were protective factor of HBV infection.Combination of rs10204525 GG and rs2227982 CC was potent protective factor of HBV infection(OR=0.552,95% CI=0.356–0.857,P=.007)and was also associated with lower HBV load(OR=0.201,95% CI=0.056–0.728,P=.008)in As C.The 4 SNP sites were not associated with progression of HBV-related liver disease.Conclusion: Rs10204525 and rs2227982 of PD-1 are associated with HBV infection.Combining these two SNP sites can better predict the host susceptibility of HBV infection.