| Objective: Endometrial damage seriously affects women's reproductive function and health of physical and mental.Currently,there are many treatments for it,but the efficacy is generally not satisfactory.Therefore,this experiment uses 95% ethanol to establish an experimental model of the injured endometrium in mice,and observes the repair of endometrium by transplanting different types of MSCs orthotopically into the uterine cavity.We aimed to provide a new ideas for the treatment of injured endometrium.Methods:1.The experimental group,the experimental control group and the normal group were set up simultaneously.In the experimental group,95% ethanol was respectively infused into the uterine cavity of mice for 10 s,15 s,20 s and 25 s.In the experimental control group,PBS was infused into the uterine cavity of mice.In the normal group,no treatments were taken.After modeling,one mouse in different group was randomly killed and uterine tissue was taken out for morphological detection.HE staining and Masson staining were used to detect the endometrial morphology and fibrosis degree of the remaining mice in the third period of estrous after the operation.2.According to the above experiments,the appropriate time points were selected to model,and respectively performed in situ transplantation of Men SCs,BMSCs,UC-MSCs and PBS.In the third estrous period after operation,the mice were killed,and HE staining was used to detect the endometrial tissue morphology and thickness then;immunohistochemistry method was used to detect the expression levels of CK18,Vimentin and Itg?V?3 in endometrial tissue;as well as CK18,Vimentin and VEGF were detected by Western blot,and the differences among the experimental groups,the experimental control group and the normal group were compared.Results: 1.It was confirmed by 95% ethanol modeling that the endometrial thickness of the 15 s group and 20 s group were significantly reduced,which was statistically different form the normal group,the experimental control group and the 10 s group.Endometrial fibrosis became worse in the 20 s group.In the 25 s group,uterine adhesions were severe and necrosis occurred;2.Endometrial thickness and related protein expression in the MSCs transplantation group were significantly better than those in the experimental control group,but weaker than those in the normal group.Among MSCs transplantation group,the intimal thickness and the expression of Itg?V?3 in immunohistochemistry showed that there was no statistical difference between the Men SCs transplantation group and the BMSCs transplantation group,but the level of Men SCs transplantation group was higher than the UC-MSCs transplantation group,the difference was statistically significant.There were no significant difference in immunohistochemistry and Western blot of CK18 and Vimentin expression between Men SCs transplantation group and BMSCs transplantation group,but they were all higher than UC-MSCs transplantation group.The expression of VEGF in Western blot show that the Men SCs transplantation group was better than the BMSCs transplantation group and the UC-MSCs transplantation group.Conclusion:1.The use of 95% ethanol for intrauterine infusion and retention for 15 seconds can successfully establish the model of injured endometrium in mice.2.Three kinds of MSCs were transplanted in situ through the uterine cavity into the injured endometrium of mice.All of them can increase the thickness of the endometrium and promote the regeneration of endometrial cells.Men SCs play the most important role in promoting endometrial regeneration and improving endometrial receptivity,and it is expected to become a new way to treat endometrial injury. |
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