Analysis Of Differences In Intestinal Microflora And Metabolomics Characteristics Of Pneumoconiosis Patients

Objective In this study,16 Sr DNA gene sequencing technology and metabolomics methods are used comprehensively,with pneumoconiosis patients as the research object,from the perspective of intestinal microflora and plasma metabolomics,to explore the mechanism of pneumoconiosis occurrence and development,and to provide new prevention and treatment of pneumoconiosis Target.Methods From the Beidaihe Coal Mine Workers Nursing Home,15 cases of pneumoconiosis were selected as the experimental group and blood and feces were collected.At the Dongpang Coal Mine Hospital in Xingtai,15 non-dust-collecting patients were selected as the control group and blood and feces were collected.16 Sr DNA gene sequencing technology was used to analyze the differences in intestinal flora of each experimental group through OTU analysis,cluster analysis,Alpha diversity analysis,flora β diversity analysis,and LEf Se analysis of samples between groups.PCA analysis,OPL-DA analysis,KEGG and other analytical methods were used to analyze the differences in plasma metabolites between the experimental groups.Results Metabolomics analysis found that plasma metabolites in the pneumoconiosis patient group were significantly different from the normal group.With fold change> 2;vip> 1;P <0.05 as the screening criteria,a total of 10 different metabolites were screened in positive and negative ion mode,namely diethanolamine(Diethanolamine),dipeptide(Leu-Leu),glutamic acid(L-Glutamate),tryptophan(L-Tryptophan),phenylacryloyl tryptophan(Phe-trp),formylanthranilic acid,eicosapentaenoic acid,muscle Inosine,Acamprosate,and Phenacemide,the above metabolites were up-regulated to varying degrees in the pneumoconiosis patient group.The metabolic pathway analysis of the differential metabolites yielded a total of 7 metabolic pathways: glycerophospholipid metabolism,D-glutamine and D-glutamic acid metabolism,cyanine metabolism,aminoacyl t RNA biosynthesis,nitrogen metabolism,purine metabolism 1.Metabolism of tryptophan.Among them,the aminoacyl t RNA biosynthesis pathway changed most obviously between the two groups.Using 16 Sr DNA technology to analyze the intestinal flora of the pneumoconiosis patient group and the normal group,it was found that compared with the healthy control group,the ACE,Chao1,Shannon and Simpson indexes of coal worker pneumoconiosis patients had no Significant difference(P> 0.05).Beta diversity analysis showed that there was a difference in microbial communities between pneumoconiosis patients and normal people.Pneumoconiosis patients Actinobacteria abundance decreased,Actinobacteria abundance decreased,abundance decreased,Selenomonadales,Veillonellaceae(Veillonellaceae)abundance decreased,Negativicutes abundance decreased,The abundance of Bifidobacteriales decreased,the abundance of Bifidobacteriaceae decreased,the abundance of Bifidobacterium decreased,the abundance of Bifidobacterium_uncultured decreased,and the abundance of Prevotellaceae increased.Conclusion 1.Compared with the normal control group,the intestinal flora of pneumoconiosis patients Actinomycetes,Actinomycetes,Veillonellaceae,Veillonellaceae,Bifidobacteria,Bifidobacteriaceae,Negativicutes,Selenomonadales,Bifidobacterium uncultured,the abundance of Bifidobacterium decreased,and the abundance of Prevotidae increased.2.Plasma metabolites of pneumoconiosis patients are compared with the healthy control group diethanolamine,dipeptide,glutamic acid,tryptophan,phenylacryloyl tryptophan,formimidic acid,eicosapentaenoic acid,Glycoside,acamprosate and phenylacetylurea were up-regulated.The metabolic pathway analysis of the differential metabolites yielded a total of 7 metabolic pathways: glycerophospholipid metabolism,D-glutamine and D-glutamic acid metabolism,cyanine metabolism,aminoacyl t RNA biosynthesis,nitrogen metabolism,purine metabolism 1.Metabolism of tryptophan.Among them,the aminoacyl t RNA biosynthesis pathway changed most obviously between the two groups.It provides a theoretical basis for further research on the pathogenesis,early prevention,and treatment of pneumoconiosis.Figure27;Table11;Reference 79...