Establishment Of Double Artery Blood Supply Model And Its Effect On Energy Metabolism Of Mitochondria In Liver Regeneration Of Rats

Objective:1.To establish a stable and successful animal model of 68%partial hepatectomy+liver dual arterial blood supply（LDABS）in rats under microscope.2.Through the establishment of the model,the liver mitochondria were separated and the influence of the dual arterial blood supply on the energy metabolism of the liver mitochondria was discussed.Methods:1.76 rats were divided into training group（40 rats）and model group（36 rats）.In the model group,68%partial hepatectomy（left lobe and middle lobe of liver）+double artery blood supply operation of liver were performed under microscope.The general situation of rats was observed after operation,and the success rate and 1-W survival rate were calculated.2.108 male and clean SD rats were randomly divided into three groups:sham operation group（SO group）,68%partial hepatectomy group（PH group）,68%partial hepatectomy combined with dual arterial blood supply group（PH+LDABS group,named LDABS group）.Each group of animal models were taken at 0,12,24,72,120 and 168 hours after operation.The effects of mitochondrial energy metabolism of LDABS were explored by extracting liver mitochondria,measuring mitochondrial membrane potential,respiratory chain enzyme concentration and ATPase analysis.Results:1.Pass the learning curve through the simulation operation of the training group.The operation time of the model group was 65.3±6.56 min,and the vascular anastomosis time was11.1±2.53 min.The bleeding volume was 0.6±0.21m L.The success rate was 100%（36/36）in 24 hours and 88.9%（32/36）in 1 week postoperatively.2.（1）Compared with the SO group,the mitochondrial membrane potential of liver tissue in the PH group increased at 12 hours after operation,decreased at 1-3 days,but there was no significant difference.Compared with the PH group,the mitochondrial membrane potential of liver tissue in the LDABS group increased at 3 days after treatment,but there was no significant difference.（2）Compared with the PH group,the water level of mitochondrial respiratory chain enzyme I in the LDABS group increased on the average from 0.5 to 7 days,and there was a significant difference between 0.5 and 3 days（P<0.05）.The level of respiratory chain enzyme II and III in the LDABS group increased on the antenna particles of 0.5-3,but there was no significant difference.（3）Compared with the PH group,the level of Ca²⁺Mg²⁺-ATPase in the LDABS group did not change significantly at 0-120h,there was no significant difference;168h after operation was significantly lower than that in the PH group,there was significant difference.Compared with the SO group,the level of Ca²⁺Mg²⁺-ATPase in the LDABS group increased significantly at 24h after operation.Compared with the SO group,the level of Ca²⁺Mg²⁺-ATPase in the PH group was significantly lower at 0h after operation.In terms of the content of Na⁺K⁺-ATP enzyme,the content of LDABS group reached the peak at 12h after operation,and then decreased in each period of time.Compared with PH group,the content of LDABS group increased significantly at 0h after operation with significant differences.Conclusions:1.The operation of 68%partial hepatectomy+LDABS model under microscope is complex,but after a certain period of microscopical skill training,the success rate of the model is high and the repeatability is strong,which lays a foundation for further study of its application in liver regeneration,acute liver failure and liver transplantation.2.LDABS plays an important role in liver regeneration through mitochondrial respiratory chain enzyme I.LDABS has a certain function of compensating energy metabolism of liver injury.LDABS can promote liver cell regeneration through energy metabolism at an earlier stage,and advance the peak of energy metabolism required for liver regeneration.