| Objective By observing the effect of rose polyphenols on the blood lipids of hyperlipidemia rats,the effect of rose polyphenols on reducing blood lipids was clarified,and the mechanism of action was explored.Method 70 healthy adult male SD rats were randomly divided into 7 groups:normal group,model group,rose polyphenol high,medium and low dose group,simvastatin group and fenofibrate group.The normal group was given normal feed every day,and the rats in the other groups were given high-fat diet every day,at the same time as required.The doses of rose polyphenol high,medium and low dose groups were140mg/kg,70mg/kg and 35mg/kg,0.9mg/kg in the simvastatin group and 27mg/kg in the fenofibrate group.Rats in the hyperlipidemia model group and normal group were given the same amount of distilled water.The intragastric volume of rats in each group was 10ml/kg,once a day for 6 weeks.After the last day of the administration,the rats were fasted for 12 h and weighed and sampled.Main detection indicators:1.Use a fully automated biochemical analyzer to detect triglyceride(TG),total cholesterol(TC),and low-density lipoprotein(LDL-C),the content of high density lipoprotein(HDL-C);2.Observe the pathological changes of liver tissue under the microscope using HE staining method;3.Detect the abdominal aorta by enzyme-linked immunosorbent assay Fatty acid synthase(FAS),Acetyl Co A carboxylase(ACC),hormone-sensitive triglyceride lipase(HSL),lipoprotein lipase(LPL)content;4.Western blot method to detect FAS,ACC,LPL,SREBP-1c(Sterol regulatory element binding protein 1c,Sterol regulatory element binding protein-1c)protein expression in liver tissue.Results 1.During the experiment,the weight of the rats in each group gradually increased.At the end of the experiment,the body weight of the model group was significantly higher than that of the normal group(P<0.05).The body weight of the rats in the fenofibrate group decreased compared with the model group,but there was no significant difference.2.Blood lipid test results showed that:compared with the normal group,the content of TG,TC,LDL-C in the serum of the model group was significantly increased,and the content of HDL-C was significantly reduced(P<0.05);The TG content of rose polyphenol high,middle-dose group and fenofibrate group decreased(P<0.05),the TG content of rose polyphenol low-dose group and simvastatin group decreased,but there was no significant difference.The content of TC and LDL-C in the simvastatin group was significantly reduced(P<0.05).3.HE staining results showed that the liver cells of the normal group had a regular shape,round nuclei and centered position,and clear cell structure.In the model group,round vacuoles of different sizes and different amounts appeared in the hepatocyte cytoplasm,and part of the hepatocyte nuclei were squeezed toward the side of the hepatocytes.The cells in the high,medium and low dose groups of roses polyphenols were not arranged regularly,the structure was not clear,the liver sinusoids were gradually clear,and no lipid droplets were seen.The nucleus position of the simvastatin group and fenofibrate group was centered,the shape was regular,and steatosis was rare.4.Elisa test results showed that:compared with the normal group,the FAS,ACC,HSL and LPL contents of the model group were significantly increased(P<0.05).Compared with the model group,the FAS enzyme content of rose polyphenol high,medium and low dose groups,simvastatin group and fenofibrate group was significantly reduced(P<0.05);rose polyphenol high dose group,Xin The enzyme content of ACC in the statin group and fenofibrate group was significantly reduced(P<0.05);the LPL enzyme content in the high-dose rose polyphenol group,simvastatin group,and fenofibrate group was significantly reduced(P<0.05);HSL enzyme content of rose polyphenol high-dose group,simvastatin group and fenofibrate group was significantly reduced(P<0.05).The comparison between rose polyphenol groups showed that the FAS content of rose polyphenol high-dose group was significantly lower than that of rose polyphenol medium and low-dose groups(P<0.05),and the FAS enzyme content of rose polyphenol medium-dose group was more Rose polyphenols in the low-dose group decreased significantly(P<0.05).The FAS enzyme content of simvastatin group and fenofibrate group was significantly lower than that of rose polyphenols high-dose group(P<0.05).The content of ACC and FAS in the simvastatin group and fenofibrate group was significantly lower than that in the rose polyphenol low-dose group(P<0.05);the content of LPL and HSL enzyme in the simvastatin group and fenofibrate group was more Rose polyphenols were significantly reduced in the low-dose group(P<0.05).5.Western blot test results showed that compared with the normal group,the expression levels of FAS,ACC,LPL and SREBP-1c protein in the liver of the model group were significantly increased(P<0.05).Compared with the model group,the protein expression levels of FAS,ACC,and SREBP-1c in the liver of each group of rats were significantly reduced(P<0.05);rose polyphenol high,middle-dose group,and simvastatin group,non-The LPL protein expression level in the Norbert group was significantly reduced(P<0.05).The protein expression levels of FAS,ACC,and SREBP-1c in the high-dose rose polyphenol group were significantly lower than those in the low-dose group(P<0.05),and significantly higher than those in the simvastatin group and fenofibrate group(P<0.05).The protein expression levels of FAS,ACC,and LPL in the rose polyphenol middle-dose group were significantly lower in the lower-dose group(P<0.05),and significantly higher than those in the simvastatin group and fenofibrate group(P<0.05).The expression levels of FAS,ACC,LPL and SREBP-1c protein in the low-dose rose polyphenol group were significantly higher than those in the simvastatin group and fenofibrate group(P<0.05).Conclusion 1.Rose polyphenols can reduce the levels of TG,ACC,and FAS in the serum of hyperlipidemic rats,thereby playing a role in lowering blood fat.2.Rose polyphenols can improve the cell structure of liver in hyperlipidemia rats and repair steatosis.3.Rose polyphenols can reduce the protein expression of the key enzymes of fatty acid synthesis,ACC and FAS,by inhibiting the activation of SREBP-1c,delaying the rate of fat synthesis,and then achieving the purpose of reducing blood lipids. |
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