Monitoring Circulating Tumor Cells In Prostate Cancer Model After Tumor Resection By In Vivo Flow Cytometry

Tumor recurrence induced by metastasis is the root cause of incurable cancer.Recently,circulating tumor cells(CTCs)has become a promising marker for metastasis and prognosis.Besides,CTCs can be detected continuously and noninvasively by in vivo flow cytometry,which has been widely applied in oncological research.During the past two decades,the incidence rate of prostate cancer in China has kept increasing and preferred treatment advised for early stage prostate cancer patients is prostatectomy.However,surgeries have been reported to have various side effects like accelerating tumor proliferation and promoting metastasis in animal studies.Thus,studying the relationship between prostatectomy and CTCs is of great significance.In this study,we applied IVFC in enumerating CTC before and after prostatectomy and we found out that tumor surgery was able to elongate the overall survival and lower CTC numbers.However,we observed more metastasis sites and tumor recurrence in surgery group in orthotopic cancer model.In conclusion,we hypothesized that surgeries might promote shedding and what's worse,unthorough resection would lead to tumor recurrence so that surgeries under this condition should be applied with enough caution.Our study evaluated the efficacy of prostatectomy in terms of the impact on CTCs and provided theoretical support for making best treatment plans for prostate cancer patients.