Drug Resistance Analysis And Investgation The Mechanism Of Carbapenemase-producing Klebsiella Pneumoniae

Objective:To provide evidence for clinical anti-infection treatment and reducing the carbapenem resistant klebsiella pneumoniae(CRKP).The carbapenem resistant strains were collected from a hospital in chongqing.The drug resistance and mechanism was analysed.Methods: Klebsiella pneumoniae strains were isolated from a hospital in chongqing from 2013 to 2018.CRKP was screened by antibiotic sensitive test and MIC.Carbapenem-producing strains were screened by modified Carba NP test.The carbapenemase-coding genes were amplified by PCR and the PCR products were sequenced and compared.Results: A total of 11 CRKP strains were collected and screened from clinical samples.Most of them were all resistant to meropenem,imipenem and ertapenem.The modified Carba NP test showed that the six strains were positive for the enzyme producing phenotype.The plasmid conjugation test showed that the drug resistance gene had horizontal transmissibility.The drug-resistant genes were amplified by PCR,and the sequencing results were compared with the gene pool.The results showedthat 4 strains of the enzyme producing strain carried the KPC gene,1strain carried the NDM gene,and 1 strain carried the VIM gene.Conclusion:The production of carbapenemase by bacterial strains is an important cause which leads to drug resistant.Also it is one of the main mechanisms of bacterial resistance.The results showed that the CRKP collected from the hospital mainly carried KPC,NDM and VIM genes.The drug-resistance bacteria could transfer the drug resistance genes to the recipient bacteria by plasmid conjugation test,suggesting that there was a certain risk of transmission of this kind of drug resistance genes.The emergence of drug-resistant bacteria has brought so many problems for clinical anti-infection treatment.