Knockdown Of Fez Family Zinc Finger Protein 1 Antisense Ribonucleic Acid 1(FEZF1-AS1) Inhibits Invasion And Migration Of Esophageal Squamous Cell Carcinoma Cells By Blocking JAK2/STAT3 Pathway

Objective:To investigate the relationship between long chain non-coding RNA Fez family zinc finger protein 1 antisense ribonucleic acid 1(FEZF1-AS1)and invasion,migration and Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3)signal pathway in esophageal squamous cell carcinoma(ESCC)cells.Methods:The expression of FEZF1-AS1 in ESCC cancer tissue and ESCC cell line was detected by real-time quantitative PCR.After knocking down the FEZF1-AS1 of KYSE150 and KYSE510 cells by the constructed lentiviral expression vector,the changes of cell cycle were detected by flow cytometry,the ability of cell proliferation was detected by colony formation assay,the ability of cell invasion was detected by Transwell~TMM invasion assay,the ability of cell migration was detected by Transwell ~TMM migration and scratch assay,and the protein levels of JAK2 and phosphorylated STAT3(p-STAT3)were detected by Western blot.Results:FEZF1-AS1 was highly expressed in ESCC cancer tissues,and FEZF1-AS1 expressions in KYSE150 and KYSE510 cells were higher than those in normal esophageal squamous epithelial cells.After the knockdown of FEZF1-AS1 expression,the cell cycle and proliferation of ESCC cancer cells had no significant change,but cell invasion and migration were significantly inhibited,and the level of JAK2 protein decreased and the level of p-STAT3 protein increased.Conclusion:knockdown of FEZF1-AS1 blocks JAK2/STAT3pathway and inhibits the invasion and migration of ESCC cells.