Study On Cell Separation And Enrichment Based On Inertial Microfluidic Technology

Currently,accompanying with the increasingly widespread application,microfluidic technology has been regarding as a crucial process by numerous industries,including including biological research,chemical analysis,environmental testing and medical diagnosis.At the same time,the number of cells which can be utilized in medical diagnosis and sorted by microfluidic technology has seen a rising trend.In the basis of maintaining cells' original morphology and activity,this paper aims to achieve certain accomplishment by using microfluidic technology mainly.The sorting of white blood cells,pathogenic bacteria and circulating tumors cells was effectively completed by adopting DFF inertial microfluidic chips with the same structure.And then the chips combined with the passive flow regulating valve are loaded into an instrument to integrate these three functions,in order to provide technical support for the commercialization of inertial microfluidic technology,and bring huge benefits to diseases diagnosis.The main research contents of this paper are as follows:(1)Sorting white blood cells,pathogenic bacteria and circulating tumors cells effectively by adopting DFF inertial microfluidic chips with the same structure.Simulation experiments was conducted to explore the size of cell-sorted chips by polystyrene particles and the functional efficiency of the chips was tested by flow cytometry,blood cell counters,and immunofluorescence.After that,the bio-compatibility test,culturing post-sorted circulating tumor cells for one more week,was implemented to see the fact whether it will impact cells' activity and do harm to subsequent biochemical analysis.(2)Exploring the specific flow rate of the high-throughput passive flow regulating valve under various structural parameters,and merging the inertial microfluidic chip to manufacture the eight-core integrated chip to complete the undiluted blood as much as possible.The studies of the relationships between the three micro-valves' primary parameters,including the shrinkage flow path height H,the shrinkage flow path width W and the control flow path width L,and flow rate is of vital importance.And then,the minimum and maximum flow rates that can be achieved under laboratory conditions are tested.Besides,the integrated microfluidic chip with eight spiral channel sorting units was fabricated to achieve high throughput processing for the sample.At the same time,in order to increase flow rate,research on low-dilution blood was carried out,and the treatment of the undiluted blood sample was completed as much as possible.(3)Using a instrument with different integrated microfluidic chips to automatically sort white blood cells,pathogenic bacteria and circulating tumors cells.The instrument parameters were adjusted,and the corresponding integrated microfluidic chip was loaded to accomplish the sorting of the three kind of samples.The efficiency of these three functions was tested by flow cytometry.As a consequence,multi-functional instrument was manufactured,thereby contributing to multi-disease monitoring.