| Polygonatum sibiricum is the dried rhizome of Liliaceae,which is a traditional Chinese medicine used for both medicine and food in China.Polygonatum contains polysaccharides,steroidal saponins,flavonoids and other components,which can enhance immunity,antioxidant,anti-tumor,regulation of blood glucose and blood lipids and other pharmacological effects.Polygonatum contains polysaccharides,steroidal saponins,flavonoids and other components,which can enhance immunity,anti-aging,antibacterial,antioxidant,anti-tumor,regulation of blood glucose and blood lipids and other pharmacological effects.In this paper,the extraction,purification,structure,biological activity and granule preparation technology of Polygonatum polysaccharide were studied.The specific results are as follows:(1)The technological parameters of enzymatic extraction of Polygonatum polygonatum polysaccharides were optimized by response surface method,and the optimum process parameters were obtained as follows: cellulase addition 2.1%,time 51 min,temperature 54?,pH 5.0,and the extraction rate of Polygonatum polygonatum polysaccharides was 29.54%.(2)The protein removal effect of acid protease was the best among the selected protein removal methods.Under the conditions of pH 3,enzymatic hydrolysis temperature 50 ?,enzymatic hydrolysis time 40 min and enzyme addition 4%,the protein removal rate was 89.12% and the polysaccharide retention rate was 94.86%.The decolorization effect of macroporous resin D101 is the best among the selected decolorization methods.Under the conditions of temperature 50?,time 2 h,resin addition 5 g/ 100 mL,the decolorization rate is 89.87%,and the polysaccharide retention rate is 81.64%.Using distilled water and 0.5 mol/L NaCl solution as eluent,PSP-1 and PSP-2 were obtained by DEAE-52 cellulose column chromatography.PSP-1S and PSP-2S were further purified by SephadexG-100 dextran gel column.(3)Infrared spectrum results show that both PSP-1S and PSP-2S have characteristic peaks of polysaccharides and have ?-type glycosidic bonds;the relative molecular weights of PSP-1S and PSP-2S determined by gel chromatography are 3.31 KDa and 3.73 KDa,respectively.The results of ion chromatography showed that PSP-1S was composed of arabinose,galactose,glucose,xylose,mannose,fructose,galacturonic acid and glucuronic acid,while PSP-2S was composed of arabinose,rhamnose,galactose,glucose,xylose,mannose,fructose,galacturonic acid and glucuronic acid,and the two components are mainly composed of glucose and galactose,in which PSP-1S contains 75.535% glucose and 12.844% galactose;PSP-2S contains 62.424% glucose and 15.842% galactose.(4)In vitro hypoglycemic study showed that PSP-1S and PSP-2S had certain hypoglycemic effect.When the mass concentration was 3 mg/mL,the inhibition rate of PSP-1S on ?-amylase activity was 49.20% and the IC50 was 3.04 mg/mL;the inhibition rate of PSP-1S on ?-Glucosidase activity was 55.90% and the IC50 was 2.27 mg/mL;the inhibition rate of PSP-2S on ?-amylase activity was 31.17% and the IC50 was 5.25 mg/mL,the inhibition rate of PSP-2S on ?-glucosidase activity was 40.17% and the IC50 was 4.13mg/mL.In vitro antioxidant study showed that PSP-1S and PSP-2S had certain antioxidant effect.When the mass concentration was 5 mg/mL,the scavenging rate of PSP-1S on DPPH free radicals was 47.31%,the IC50 was 6.51 mg/ml,and the antioxidant equivalent was 4.20 umol TE/1 mg PSP,the scavenging rate of PSP-1S on ABTS free radicals was 50.48%,the IC50 was 5.14 mg/mL,and the antioxidant equivalent was 4.41 umol TE/1 mg PSP;the scavenging rate of PSP-2S on DPPH free radicals was 38.30%,the IC50 was 11.84 mg/mL,and the antioxidant equivalent was 3.40 umol TE/1 mg PSP;the scavenging rate of PSP-2S on ABTS free radical was 43.49%,the IC50 was 7.65 mg/mL and the antioxidant equivalent was 3.80 umol TE/1 mg PSP.(5)The optimum granulation conditions are as follows: the ratio of raw materials and auxiliary materials is 1: 2,the mixture of soluble starch and mannitol is 1: 1,85% ethanol as wetting agent.The prepared particles can be completely dissolved in hot water in 5 min,,the total amount of Pass through No.1 sieve and can't pass No.5 sieve was 6.52% < 15%.,and the moisture content is 5.31% < 8%,which meets the requirements of the 2015 edition of Chinese Pharmacopoeia. |
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