| Objective: To investigate the differences in the expression of cold sensitive receptor transient receptor potential channel melastatin 8(TRPM8)between chronic obstructive pulmonary disease(COPD)and healthy people in nasal mucosa;to analysis the correlation of TRPM8 mRNA expression level and clinical characteristics in COPD group.Methods: 17 cases of nasal mucosal tissue samples were collected(10 in the normal control group and 7 in the COPD group),immunohistochemistry and western-blot were used to detect the difference of TRPM8 expression between the two groups.79 cases of nasal mucosa brushing cells were collected(30 in the normal control group and 49 in the COPD group),quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of TRPM8 mRNA in nasal mucosal epithelial cells in two groups,and the patients in the COPD group were divided into two subgroups: high TRPM8 expression and low TRPM8 expression.The differences in clinical characteristics between the two subgroups were compared and statistically analyzed.Results: TRPM8 was expressed in nasal mucosa of all subjects,and the expression level of TRPM8 protein in COPD group was significantly higher than that in healthy subjects(p<0.05);The times of annual acute attacks,the degree of impairment of basal lung function,the COPD assessment test(CAT),the number of patients sensitively to cold stimuli in high TRPM8 expression group were significantly higher than those in the low TRPM8 expression group,however,the degree of lung function recovery and the improvement of symptoms after inhalation of long-acting β2-agonists(LABA)or /and long-acting muscarine anticholinergic(LAMA)were worse than those of low TRPM8 expression group(all p<0.05).Conclusion: The high expression of cold sensitive receptor TRPM8 in the nasal mucosa of COPD patients may be an important factor for COPD to be highly sensitive to cold stimuli and lead to the development of the disease. |
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