Cardiomyocyte Culture Based On Anisotropic Inverse Opal Film

The inverse opal structure has become one of the research focuses in the field of tissue engineering due to its flexible and controllable micro-nano structure and unique optical properties.The inverse opal substrate has uniform pores and interconnected windows,which facilitate cell distribution,differentiation and diffusion of biological macromolecules.As a biomedical biomimetic scaffold,its highly adjustable and precisely controlled properties will lead to a paradigm shift in the study of tissue engineering and regenerative medicine.In addition,inspired by nature's flora and fauna,artificial actuators have been extensively studied in response to changes in external environmental signals such as light,electricity,magnetism,heat,p H and humidity.By integrating self-driving cells with inverse opal substrates,biohybrid actuators without complicated and cumbersome equipment can be fabricated,which can also generate intuitive visual response signals to complex environmental signals,presenting great potential in biosensing and tissue engineering.Based on the vertical deposition method for colloidal crystal templates,this paper developed anisotropic inverse opal films and constructed biohybrid actuators to realize the design of bionic guppy,the construction of cardiac chips and the assessment for cardiac drugs.the specific work is as follows:(1)Preparation of polymer films based on inverse opal structure: The periodic tightly packed silica templates were prepared by vertical deposition method,and the inverse opal films with periodic pores were prepared by using polyvinylidene fluoride as raw material.The structural characteristics and optical properties of the inverse opal film were explored and the construction of the dual response sensor was realized.(2)Preparation of anisotropic inverse opal film and its effect on cardiomyocytes: Inverse opal films were prepared by template sacrificial method.Then,it was stretched by an external force in a water bath,obtaining a periodic elliptical macroporous structure.With hydrogel filled,this anisotropic film presented high biocompatibility and could induced cardiomyocyte into a highly ordered alignment,providing technical support for tissue sensing and cardiac chips.(3)Based on the biohybrid actuators to construct the biomimetic system and the heart chip: By culturing the cardiomyocytes on the anisotropic inverse opal films,the biohybrid actuators were constructed and the bionic guppy with self-wave function was prepared.In addition,the biohybrid actuators were integrated into the microfluidic device to obtain the cardiac chip,realizing the real-time optical feedback on the frequency and amplitude of cardiomyocytes beating,and the evaluation of cardiac drugs.