The Preparation And Biological Properties Study Of Polyetheretherketone(PEEK) Surface-modified By Poly(sodium P-styrene Sulfonate)(pNaSS) Via Ultraviolet-induced Grafting Polymerization

Objective:In this study,poly(sodium p-styrene sulfonate)(pNaSS),a polymer with osteogenic property,was grafted onto the surface of polyether ether ketone(PEEK)by ultraviolet(UV)irradiation.In vitro cell experiments were conducted to explore the osteogenic promotion ability of pNaSS modified PEEK,providing a simple and efficient modification program for PEEK as a bone implant material.Method:pNaSS with different graft amounts were introduced into the PEEK surface by UV irradiation.Samples were divided into group P-1,P-2,P-3,P-4,P-5 and control group P-0 according to the graft amounts.The surface properties and morphology of samples in different groups were tested and observed by Fourier transform infrared spectrometer(FTIR),contact Angle(CA)and scanning electron microscopy(SEM)tests.Toluidine blue(TB)staining was used to quantitatively test the grafted polymer on the surface of the material.The influences of surface modification on mechanical properties of materials were evaluated by mechanical tensile test.In vitro mineralization experiments were conducted to test the effect of graft on mineral deposition on the surface of samples.MC3T3-E1 osteoblasts,a model cells in vitro study were used to observe the cytotoxicity of the materialthe and the adhesion,proliferation and differentiation abilities of osteoblasts on the surface of each group of samples.Results:Various material characterization tests and mechanical property test results show that by adjusting the UV irradiation time and monomer concentration,the amount of grafted pNaSS on the PEEK surface could be well-controlled from 0.59±0.07mmol/cm~2 to 5.08±0.20 mmol/cm~2.With the increase of grafting account,the hydrophilicity of the material surface gradually increased,and the mineralization ability in vitro also increased significantly.Besides,this surface modification method did not influence the intrinsic mechanical properties of PEEK.In vitro biological studies revealed that the grafted material has good cellular compatibility and cell adhesion,proliferation,osteogenic differentiation of MC3T3-E1 cells were enhanced with the increase of graft amount.Conclusion:In this study,pNaSS was successfully grafted on the PEEK surface by UV irradiation.While the excellent mechanical properties of PEEK were not changed,graft modification effectively improves the adhesion,proliferation and differentiation of osteoblasts.