Study On The Relationship Between The Inhibition Of Proliferation Of SW620 By Tetrandrine And BMP9/PTEN

Although the mortality of colon cancer is generally declining in the entire population,there are still many people suffering from this disease in the world.So it is still of great concern to find effective treatment drugs or targets.We studied Tetrandrine in this project,which was extracted from the dried root of Chinese medicine,especially its inhibitory effect on colon cancer cells and its mechanism analysis.In this study,we used cell viability assays,flow cytometry analysis,and colony formation experiments,and confirmed that Tet can inhibit SW620 cell proliferation,induce cell cycle arrest and apoptosis.As for mechanism,recombinant adenovirus,real-time fluorescent quantitative PCR,immunofluorescence and western blotting were used.And it was confirmed that Tet can up-regulate the m RNA and protein levels of BMP9 in SW620 cells;over-expressed BMP9 enhanced the inhibition proliferation effect of Tet in SW620 cells,but these effects can be partially reversed by silencing BMP9;and Tet reduced the protein level of phosphorylated Aktl/2/3 in a concentration-dependent manner in SW620 cells.Over-expressed BMP9 can intensify this effect of Tet.However,silencing BMP9 can weaken this effect,so it is speculated thatthis may be caused by Tet up-regulating the protein level of BMP9.In addition,we demonstrated that Tet can reduce the phosphorylation level of PTEN,and that over-expressed BMP9 enhances the anti-proliferation effect of PTEN in SW620,similarly,silencing BMP9 reduces its effect.Finally,we introduced a xenograft tumor model to study the antiproliferative effect of Tet,and further explored the role of BMP9 and PTEN in SW620 cells.Our findings suggested that the anti-cancer activity of Tet in SW620 cells may be mediated partly by up-regulating BMP9,followed by inactivation PI3K/Akt through up-regulating PTEN at least.