| Objective:To investigate the expression of HMGB1?UCHL1 in serum and hippocampus of young rats with epilepsy induced by pentylenetetrazole?PTZ?,and the effects of mouse nerve growth factor?mNGF?and LPS-RS on it's expression,and to explore the intervention effect of mNGF and LPS-RS on nerve damage caused by epilepsy.Methods:130 Clean grade Wistar male rats,21 days old were randomly divided into:group A?Blank control group,n=31?,group B?EP group,n=33?,group C?mNGF group,n=33?,group D?LPS-RS group,n=33?.Mode of administration was intraperitoneal injection?IP?.During the modeling period,group A was IP an equal amount of 9 g·L-1normal saline per day,and the other groups were IP PTZ 40 mg·kg-1.Intervention was performed after 14 days of administration to establish a chronic epilepsy model.During the intervention period,groups A and B were IP an equal amount of 9 g·L-1 normal saline,group C was IP with mNGF 4?g·kg-1,group D was IP with LPS-RS 0.1mg·kg-1for 7 days.Each rat was observed for 1 hour after administration and its behavioral performance was recorded.After the last intervention,9 specimens were randomly collected from each group at 3h,24h,and 72h.The expression of UCHL1 in serum and hippocampus and the concentration of HMGB1 in serum were detected by ELISA,and the content of HMGB1 in hippocampus was detected by Western Blot.Results:1.Behavioral observation:in addition to group A,after intraperitoneal injection of pentylenetetrazole,mice in groups B,C,and D showed various degrees of seizures.After 14 days of kindling,the model of chronic epilepsy was successfully established.2.ELISA test results:The results of serum HMGB1 showed that the concentration of serum HMGB1 in group A was the lowest and that in group B was the highest.the concentration of serum HMGB1 in group C was significantly higher than that in group D?all P<0.05?.The detection of serum UCHL1 showed that the concentration of serum UCHL1 in group A was the lowest,the concentration of serum UCHL1 in group B was the highest,and the concentration of serum UCHL1 in group C was higher than that in group D.There was significant difference in each group at each time point?all P<0.05?;The results of hippocampal UCHL1 showed that the change trend of hippocampal UCHL1 was similar to that of serum UCHL1,the concentration of hippocampal UCHL1 in group A was the lowest,and the concentration of hippocampal UCHL1 in group B was the lowest.The concentration of UCHL1 in hippocampus of group C was higher than that of group D.There was significant difference in each group at each time point?all P<0.05?.3.The results of Western Blot showed that the content of HMGB1 in hippocampus of group B,C and D was significantly higher than that of group A at each time point?all P<0.05?,and the content of HMGB1 in hippocampus of group C and D was significantly lower than that of group B?all P<0.05?.The content of HMGB1 protein in hippocampus of group C was significantly higher than that of group D.The difference was statistically significant?all P<0.05?.Conclusions:1.HMGB1 may play a key role in the occurrence and development of seizures.2.Serum UCHL1 may be a marker of brain damage associated with epilepsy.3.mNGF has a repairing effect on nerve injury caused by epilepsy and a protective effect on cerebral nerve.4.LPS-RS may inhibit the HMGB1/TLR4 signaling pathway,blocking the immune inflammation of the nervous system caused by neurons glial cell activation,so as to effectively reduce the brain damage caused by epileptic seizures. |
PDF Full Text Request