Relationships Between Concentrations Of Ferritin In Placenta,Serum And Umbilical Cord Blood And Oxidative Damage Levels In Placenta And Preeclampsia

ObjectiveThe purpose of this study was to investigate the relationships between concentrations of Ferritin in in placenta,serum and umbilical cord blood and oxidative damage levels in placenta and preeclampsia.MethodsA total of 60 women with preeclampsia and 60 normal pregnant women as control participated in this study.All are admitted to Fujian Maternity and Child Health Hospital for delivery from December 2017 to December 2018.Patients with preeclampsia were divided into early-onset group(n=30,presented at ?34 weeks of gestation)and lateonset group(n=30,presented at>34 weeks of gestation),with 30 normal pregnant women as early-onset control group(?34 weeks of gestation)and 30 as late-onset control group(>34 weeks of gestation).The automatic biochemical immunoassay system was used to detect the ferritin content in fetal cord blood and maternal serum;expression levels of Ferritin and FTH were detected by Reverse transcription–qPCR(RT-qPCR);the expression level of Ferritin?FTH and apoptotic protein in the placenta tissue was detected by Western Blot;TNUEL was used to calculate the apoptotic rate of cells in placenta;the content of total superoxide dismutase(T-SOD),malondialdehyde(malondialdehyde,MDA)and Glutathione Peroxidase(Glutathione Peroxidase,GSH-Px)in placenta were detected by colorimetric method.Results1.The levels of serum ferritin were(60.82±38.14)?g/L in early-onset PE group and(35.94±21.36)?g/L in late-onset PE group.Significantly elevated as compared to(27.22±23.13)?g/L mmol/L in early-onset control group and(21.50±15.83)?g/L in lateonset control group;2.The levels of cord blood serum ferritin were significantly increased in the earlyonset PE group and late-onset PE group as compared to late-onset control group;3.The levels of sTfR were significantly increased in the early-onset PE group and late-onset PE group as compared to their respective control groups;4.RT-qPCR and Western Blot revealed that: the level of Ferritin mRNA expression was significantly lower in early-onset and late-onset preeclampsia groups than lateonset control group.Western Blot revealed that the level of cleaved caspase-3 and Bax expression were significantly higher in early-onset and late-onset preeclampsia groups than late-onset control group;the level of Bcl-2?Ferritin and FTH expression were significantly lower in early-onset and late-onset preeclampsia groups than late-onset control group.5.The levels of GSH-Px and MDA were significantly elevated in the early-onset PE group and late-onset PE group as compared to late-onset control group.The levels of T-SOD expression were significantly lower in early-onset and late-onset preeclampsia groups than late control group.6.The apoptotic rates were significantly higher in early-onset and late-onsetpreeclampsia groups than late-onset control group.Conclusion1.High levels of oxidative damage in placenta may be involved in the pathogenesis of preeclampsia.2.Increased apoptosis may be one of the morphological manifestations of damaged placental tissue.3.Iron metabolism disorder may be involved in the pathogenesis of preeclampsia.