Ptni Nanocubes-catalyzed Tyramine Signal Amplification Electrochemiluminescence Sensor For Nonenzymatic And Ultrasensitive Detection Of Hepatocellular Carcinoma Cells

Hepatocellular carcinoma?HCC?is a life-threatening malignant tumor with high cancer mortality,therefore it is particularly important to sensitively detect the biomarkers of hepatocellular carcinoma for early diagnosis and treatment.Although tumor resection or liver transplantation is the most effective method for the treatment of HCC,but the high rate of postoperative recurrence and metastasis seriously affect the effect of treatment.In the early stage of liver cancer,some patients had tumor cells entering the blood circulation system to form circulating tumor cells?CTCs?.CTCs are the primary conditions that cause recurrence and metastasis of hepatocellular carcinoma after operation.Therefore,the detection of circulating tumor cells is of great significance for judging the metastasis and recurrence of liver cancer and guiding clinical treatment.In this work,a nonenzymatic and ultrasensitive electrochemiluminescence?ECL?cytosensor for HepG2 cells detection is established based on the PtNi nanocubes-catalyzed tyramine signal amplification.PtNi nanocubes?PtNi NCs?are firstly reported to catalyze tyramine-luminol?Tyr-Lum?with the aid of H₂O₂ for covalently binding to membrane proteins of HepG2 cells,realizing the introduction of massive Tyr-Lum ECL luminophores for signal amplification.Besides,PtNi NCs could also enhance the ECL signal of luminol-H₂O₂ system.On the basis of bifunctional PtNi NCs,the developed ECL cytosensor for HepG2 cells detection shows a higher sensitivity.This proposed enzyme-free-mediated tyramine signal amplification inspires a new strategy for applications in the early diagnosis of cancer cells.