| Objective :To observe the effect of adipocyte hormone leptin on GABA content and receptor expression in hypothalamus of sleep deprived mice,and to explore the possible mechanism.Methods :The male C57BL/6 mice were randomly divided into three groups: the control group(Con group),the sleep deprivation group(SD group)and the leptin supplement group(L-SD group).The control group was set in a water environment without depriving sleep,the sleep deprivation group was established by The modified multiple platform method,and the leptin supplement group(L-SD group)was given leptin 1.3mg/(kg·d)intraperitoneally at the same time.After 7 days of sleep deprivation,the general condition of the mice was observed,the body weight was measured,and hypothalamic tissue and plasma samples were collected.The levels of plasma leptin,the contents of hypothalamus GABA and Glu,and theexpressions of GABA receptor GABAARα1 protein and GABA key synthetase GAD67 protein in hypothalamus tissue were detected.Results:Compared with the control group,sleep-deprived mice lost weight significantly(P < 0.001),their fur lost their smoothness,they were particularly sensitive to light and sound,slight mania and were aggressive;The level of plasma leptin in the sleep deprivation group was significantly reduced(P < 0.01).GABA levels in the hypothalamus were lower than those in the control group,and Glu levels were increased(P<0.01,P<0.05).Exogenous leptin supplementation significantly increased hypothalamus GABA levels in sleep deprived mice(P < 0.05),and increased expression of GAD67 and GABAARα1 proteins in hypothalamus of sleep deprived mice(P<0.05);Conclusion:Leptin can up-regulate the expression of GAD67,the key enzyme of GABA synthase in hypothalamus,increase the content of GABA in hypothalamus and increase the expression of GABAAR α 1 protein in hypothalamus of sleep-deprived mice,which may be an important mechanism of leptin affecting sleep. |
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