Devices For Electrical Stimulation And Physiological Signal Acquisition Of Cardiomyocytes: Construction And Application

In myocardial tissue engineering,in vitro functional culture of cardiomyocytes and tissues has become the core of cardiac tissue engineering and the necessary technical basis for the cardiac tissue engineering industry.In order to achieve in vitro culture of cardiomyocytes and tissues,it is necessary to provide stimulation conditions similar to those in the in vivo cardiomyocyte growth environment.Furthermore,in order to characterize their physiological properties,it is necessary to collect corresponding physiological signals.In this study,an electrical stimulation system capable of applying parallel electric field stimulation to cardiomyocytes was successfully constructed,and digital electrical signals were input.The electrical stimulation system was used for long-term culture and characterization of SD rats,and the promotion of myocardial cell maturation by electrical stimulation was verified.Moreover,a set of dynamic video analysis software for beating cardiomyocytes was designed and developed to evaluate the pulsation direction,rate and pulsation area of cardiomyocytes.Furthermore,a composite chip system integrating microfluidic perfusion culture,cell electrical stimulation and electrical signal acquisition was designed and developed,the two-electrode system and micro-system are adopted,and the chip system was applied to perfusion culture and extracellular electrical signal detection of rat cardiomyocytes.In this thesis,several sets of electrical stimulation and physiological signal acquisition systems for cardiomyocytes have been developed,which provide a basis for further research on heart-on-a-chip construction and drug testing.