Effect Of MiR-708 On Oxidative Damage Of Retinal Pigment Epithelial Cells

Purpose:MicroRNA is a class of non-coding RNA molecules that play an important role in RNA silencing and post-transcriptional regulation during gene expression.The purpose of this research is to investigate the expression changes of miR-708 in human retinal pigment epithelial cells after oxidative damage,and its effects on the survival of retinal pigment epithelial cells and its gene expression.Methods:MiR-708 mimics or inhibitor was transfected into the cultured ARPE-19 cell line.ARPE-19 cells were exposed to different concentrations of H₂O₂ to mimic oxidative damage.Subsequently,the cell viability was detected by CCK-8 test,the intracellular reactive oxygen species level was detected by ROS flow cytometry analysis,the apoptosis and necrosis levels were analyzed by Hoechst 33342/PI staining and Annexin V-FITC/PI flow cytometry,and the expression level of miR-708and its target gene was detected by polymerase chain reaction.Result:After stimulation with H₂O₂,ARPE-19 cells showed a significant increase in miR-708 expression,decreased cell viability,increased intracellular ROS levels,and increased levels of apoptosis and necrosis.The addition of miR-708 inhibitor can relieve the H₂O₂-induced decrease of cell viability,decrease the level of ROS,and decrease the apoptosis and necrosis of cells.MiR-708 reduces the expression levels of multiple mRNAs such as BMI1 that reduce intracellular ROS production.Conclusion:MiR-708 may aggravate oxidative stress damage in RPE cells by regulating ROS-related genes,and miR-708 inhibitor may be a new therapeutic target for retinal degenerative diseases such as age-related macular degeneration.