Clinical Study Of Botulinum Toxin A In The Treatment Of Androgenic Alopecia

Background: Androgenic Alopecia(AGA)commonly known is as male pattern baldness,which is the most common type in male progressive hair loss.The development of androgen alopecia depends on the interactions of endocrine factors and genetic susceptibility.Androgen alopecia is characterized by progressive follicular miniaturization,with terminal hair transforming into intermediate hair,followed by villi.But the number of hair follicles per unit area remains constant,due to the androgen effect on genetically susceptible hair follicle epithelial cells in androgen-dependent areas.Although the exact pathogenesis of androgen alopecia is unknown,studies suggest that it is a polygenic disease.Oral Finasteride Tablets and topical minoxidil are the only FDA-approved drugs for AGA.There have been some reports on the attempt of botulinum toxin in AGA treatment,and its safety and effectiveness have been confirmed.However,no scientific prospective studies have been reported on injection dose,effect evaluation and side effect evaluation.Methods: 28 eligible male pattern baldness patients were recruited.In zone 1,botox A was injected with 1u/cm squared,and in zone 2,botox A was injected with 0.2u/cm squared.Patients were clinically assessed and reassessed at 4 and 8 weeks..Patients were evaluated clinically and photographs were taken before treatments.They were evaluated at 4 weeks and 8weeks,and the photos were repeated.At the same time,we established a mental stress model mouse model,and took C57BL/6 male mice and divided them into 4 groups(0u/ml,5u/ml,25u/ml,50u/ml)to study the effect of different levels of botulinum toxin concentration on apoptosis by flow experiment.Results: 28 subjects were evaluated after 4 weeks of treatment and 8 weeks after treatment.Patients in zone 1 and 2 showed improvement and there was no statistical difference between the two groups(P>0.05)..The apoptosis rate of mouse whisker hair follicle cells was significantly decreased in the medium with botulinum toxin A concentration of 25u/ml and 50u/ml.At the same time,the proliferation rate of human hair follicle papilla stem cells was significantly increased in the culture medium with the concentration of botulinum toxin A of 10u/ml and 50u/ml.Conclusion: botulinum toxin A can promote hair growth by regulating the growth cycle of hair follicle cells.